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Purified anti-ATF7 Antibody

Pricing & Availability
Clone
W16152A (See other available formats)
Regulatory Status
RUO
Other Names
Cyclic AMP-dependent transcription factor ATF-7, Transcription factor ATF-A
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16152A_PURE_ATF7_0_WB_012419
Whole cell extracts (15 µg protein) from HEK293 cells transfected with non-targeting control siRNA (siCON) or siRNA targeting ATF7 (siATF7) were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-ATF7 Antibody, clone W16152A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W16152A_PURE_ATF7_0_WB_012419
    Whole cell extracts (15 µg protein) from HEK293 cells transfected with non-targeting control siRNA (siCON) or siRNA targeting ATF7 (siATF7) were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-ATF7 Antibody, clone W16152A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W16152A_PURE_ATF7_1_WB_101917.png
    Total cell lysates (15µg protein) from MCF-7 (negative control), HeLa and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 2µg/mL purified anti-ATF7 (clone W16152A) antibody (upper). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: Molecular Weight ladder.
  • W16152A_PURE_ATF7_2_IF_101917.png
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.5 µg/ml anti-ATF7 antibody (clone W16152A) overnight at 4° followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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697302 100 µg $282
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Description

Activating Transcription Factor 7 (ATF7) is a member of the ATF/cAMP response element-binding protein family. Among these ATF family members, ATF7 shares highly structurally conserved N-terminal activation domain and the C-terminal DNA binding/dimerization domain with ATF2. ATF7 has no intrinsic transcriptional activity and forms functional heterodimers with c-Jun or c-Fos proteins. Upon stimulation, such as pathogen infection and various stresses, ATF7 is phosphorylated by stress‐activated protein kinase p38 at Thr51 and Thr53 and in turn resulted in the activation of ATF7 target genes. In the absence of stress, ATF silences targets genes by recruiting histone methyltransferases, leading to the formation of heterochromatin. ATF7 deficient mice die soon after birth. Double knockout mutations of ATF2 and ATF7 lead to prenatal lethality with abnormalities in liver and heart.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 2.0 µg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2687154 (BioLegend Cat. No. 697302)

Antigen Details

Structure
494 amino acids with a predicted molecular weight of 53 kD. Contains a N-terminal transactivation domain and a C-terminal bZIP DNA binding domain.
Distribution

Nucleus

Function
ATF7 is a transcription factor belonging to the the ATF/cAMP response element-binding protein family. ATF7 is involved in early cell signaling and is activated in response to pathogen infection and stress.
Interaction
Forms a homodimer or a heterodimer with c-Jun, c-Fos, and other ATF family proteins. Interacts with JNK2, TAF12, TAF4, and MAPK9.
Biology Area
Cell Biology
Antigen References

1. Yoshida K, Ishii S. 2016. Cell Cycle. 15:3.
2. Yoshida K, et al. 2015. Nat. Immunol. 16:1034.
3. Schaeffer E, et al. 2015. Cell Cycle. 14:2655.
4. Shivers RP, et al. 2010. PLoS Genet. 6:e1000892.
5. Camuzeaux B, et al. 2008. J. Mol. Biol. 384:980.
6. Breitwieser W, et al. 2007. Genes. Dev. 21:2069.

Gene ID
11016 View all products for this Gene ID
UniProt
View information about ATF7 on UniProt.org
Go To Top Version: 2    Revision Date: 06/11/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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