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Purified anti-ATG1 (ULK1) Antibody

Pricing & Availability
Clone
W18004A (See other available formats)
Regulatory Status
RUO
Other Names
Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1
Isotype
Rat IgG2a, κ
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Product Citations
publications
W18004A_PURE_ATG1_Antibody_1_071919
Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) of purified anti-ATG1 (ULK1) antibody, clone W18004A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W18004A_PURE_ATG1_Antibody_1_071919
    Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) of purified anti-ATG1 (ULK1) antibody, clone W18004A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W18004A_PURE_ATG1_Antibody_2_071919
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2 µg of purified rat IgG2a, κ isotype control antibody (Cat. No. 400502) or purified anti-ATG1 (ULK1) antibody, clone W18004A. The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of ATG1. Lane M: Molecular weight marker.
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622901 25 µg $101
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622902 100 µg $253
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Description

ATG1/ULK1 is a Serine/Threonine protein kinase which forms a complex with the Atg13 and FIP200 proteins. This complex is the most upstream component of the core autophagy machinery, and is therefore the key initiator of autophagy in mammalian cells. AMPK positively regulates autophagy under nutrient starvation and cellular stresses by phosphorylating ULK1, leading to the activation of the autophagic process and inhibition of mTOR. mTOR plays a negative role in this process when under growth factor- and nutrient-replete conditions, as it phosphorylates ULK1 and disrupts the interaction between ULK1 and AMPK. Autophagy is a mechanism used by tumors to survive energy deprivation and chemotherapy mediated stress. Autophagy is also implicated in autoimmune (Crohn's disease) and neurodegenerative diseases. Because of its role in autophagy ULK1 is considered an attractive drug target for such diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human ATG1 synthetic peptide conjugated to KLH (1-16 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

At higher antibody concentrations, this clone may produce minor non-specific bands between 150 and 250 kD.

 

This clone was not tested for ICC.

RRID
AB_2814476 (BioLegend Cat. No. 622901)
AB_2814476 (BioLegend Cat. No. 622902)

Antigen Details

Structure
ATG1/ULK1 is a 1050 amino acid protein with a predicted molecular weight of 112 kD.
Distribution

Cytosolic

Function
Serine/Threonine Kinase/Autophagy regulator
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Kim J, et al. 2011. Nat. Cell. Biol. 10.1038/ncb2152.
  2. Hokosawa N, et al. 2009. Mol. Biol. Cell. 20:7.
  3. Egan D, et al. 2011. Autophagy. 7:643-4.
Gene ID
8408 View all products for this Gene ID
UniProt
View information about ATG1 on UniProt.org
Go To Top Version: 1    Revision Date: 07/19/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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