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Purified anti-ATP1A1 Antibody

Pricing & Availability
Clone
A19182B (See other available formats)
Regulatory Status
RUO
Other Names
Sodium/potassium-transporting ATPase subunit alpha-1 (ATP1A1), Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATPase Na+/K+ transporting subunit alpha 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A_A19182B_PURE_ATP1A1_Antibody_032822
Whole cell extracts (15 µg total protein) from A-431 and NIH/3T3 were boiled at 37°C for 10 minutes and were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-ATP1A1 (clone A19182B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • A_A19182B_PURE_ATP1A1_Antibody_032822
    Whole cell extracts (15 µg total protein) from A-431 and NIH/3T3 were boiled at 37°C for 10 minutes and were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-ATP1A1 (clone A19182B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • B_A19182B_PURE_ATP1A1_Antibody_2_032822
    IHC staining of purified anti-ATP1A1 (clone A19182B) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm
  • C_A19182B_PURE_ATP1A1_Antibody_3_032822
    ICC staining of purified anti-ATP1A1 (clone A19182B) on A-431 cells. The cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) and permeabilized with ice cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 2.5 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 60X objective. Scale bar: 50 µm
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604751 25 µg $110
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604752 100 µg $293
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Description

ATP1A1 is part of the P-type cation transport ATPases. It is the α 1 subunit of Na+/K+ ATPase which catalyzes the hydrolysis of ATP and the exchange of Na+ and K+ maintaining their balance across the plasma membrane. In addition to regulating membrane homeostasis, the α subunit (ATP1A1) particularly plays a role in mediating viral infection, e.g., coronavirus and respiratory syncytial virus. Mutations in this gene has been associated with Charcot-Marie-Tooth disease type 2DD (CMT2DD), and Hypomagnesemia, Seizures, and Mental Retardation 2 (HOMGSMR2).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 0.5 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC, we recommend 4% PFA fixation followed by permeabilization with ice cold methanol, or fixation/permeabilization with ice cold methanol alone. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X.

RRID
AB_2924609 (BioLegend Cat. No. 604751)
AB_2924609 (BioLegend Cat. No. 604752)

Antigen Details

Structure
ATP1A1 can be processed by alternative splicing into 4 different isoforms; isoform 1 is a 1023 aa protein with a predicted molecular weight of 113kD.
Distribution

ATP1A1 is widely expressed in the plasma membrane of most tissues.

Function
ATP1A1 is an ATPase involved in maintaining the balance of Na+ and K+ across of the plasma membrane.
Biology Area
Cell Biology, COVID-19
Molecular Family
Enzymes and Regulators, Ion Channels
Antigen References
  1. Takase S, et al. 2017. Sci Rep. 7:2002.
  2. Wu IC, et al. Oncotarget. 2016 Dec 20;7(51):85244-85258
  3. Schlingmann KP, et al. 2018. Am J Hum Genet. 103:808-816.
  4. Zacherl S, et al. 2015. J Biol Chem. 290:3654-65.
  5. Azizan EA, et al. 2013. Nat Genet. 45:1055-60.
Gene ID
476 View all products for this Gene ID
UniProt
View information about ATP1A1 on UniProt.org
Go To Top Version: 1    Revision Date: 03/28/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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