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Purified anti-Bcl-2 Antibody

Pricing & Availability
Clone
100 (See other available formats)
Regulatory Status
RUO
Other Names
B-Cell CLL/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50
Isotype
Mouse IgG1
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Product Citations
publications
100_Bcl-2_Purified_Antibody_WB_121313.jpg
Western blot analysis of extracts from THP-1(lane 1), PBMC(lane 2) and Raw264.7(lane 3) cells using anti-Bcl-2 antibody(clone100). β-actin was used as loading control.
  • 100_Bcl-2_Purified_Antibody_WB_121313.jpg
    Western blot analysis of extracts from THP-1(lane 1), PBMC(lane 2) and Raw264.7(lane 3) cells using anti-Bcl-2 antibody(clone100). β-actin was used as loading control.
  • 100_PURE_Bcl-2_Antibody_IHC-P_062321.png
    IHC staining using purified anti-Bcl-2 antibody (clone 100) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 0.1 M Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (yellow) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scale bar: 50 µm
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658701 25 µg $112
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658702 100 µg $206
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Description

Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. It forms homodimers or heterodimers with other Bcl-2 family members. This protein regulates apoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin. Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Pezzella F, et al. 1993. New Eng. J. Med. 329:690. (IHC)
  2. Pezzella F, et al. 1990. Am. J. Pathol. 137:225. (IHC)
  3. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  4. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Okimoto T, et al. 2020. Cancer Sci. 111:1910. PubMed
  2. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  3. Fischer JR, et al. 2023. Cell Rep Med. 4:100977. PubMed
  4. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  5. Kartika ID, et al. 2021. Cancer Med. 10:8988. PubMed
  6. Wang F, et al. 2020. Int J Oncol. 57:707. PubMed
  7. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  8. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  9. Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
  10. NULL, et al. 2022. Cell. 185:916. PubMed
  11. Jiang Q, et al. 2022. Stem Cells Int. 2022:5897194. PubMed
  12. Chong SJF, et al. 2020. Nucleic Acids Res. 48:12727. PubMed
  13. Zhou X, et al. 2019. Med Sci Monit. 25:836. PubMed
  14. Wu YH, et al. 2020. Mol Med Rep. 21:851. PubMed
  15. Inao T, et al. 2019. Cancer Sci. 110:2690. PubMed
  16. Lu IT, et al. 2022. Mar Drugs. 20:. PubMed
RRID
AB_2562958 (BioLegend Cat. No. 658701)
AB_2562958 (BioLegend Cat. No. 658702)

Antigen Details

Structure
Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.
Distribution

Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.

Function
Regulates apoptosis through controlling mitochondrial fusion and fission.
Interaction
BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References

1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243.
2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7.
3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414.
4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571.
5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852.

Gene ID
596 View all products for this Gene ID
UniProt
View information about Bcl-2 on UniProt.org
Go To Top Version: 2    Revision Date: 06/23/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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