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Purified anti-Bcl-XS/L Antibody

Pricing & Availability
Clone
2H12 (See other available formats)
Regulatory Status
RUO
Other Names
Apoptosis regulator Bcl-X, Bcl-2-like 1 protein
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
2H12_Pure_BcL-XSL_Antibody_1_082118
Total cell lysates (15 µg protein) from PC3 (lane 1), HeLa (lane 2), HaCaT (lane 3), 293E (lane 4) and NIH3T3 cells (lane 5) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of Purified anti-Bcl-XS/L Antibody, clone 2H12 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for Purified anti-Bcl-XS/L Antibody or 1:5000 diluted Direct-Blot™ HRP anti-GAPDH Antibody, clone W17079A (lower panel). Lane M: Molecular weight ladder.
  • 2H12_Pure_BcL-XSL_Antibody_1_082118
    Total cell lysates (15 µg protein) from PC3 (lane 1), HeLa (lane 2), HaCaT (lane 3), 293E (lane 4) and NIH3T3 cells (lane 5) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of Purified anti-Bcl-XS/L Antibody, clone 2H12 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for Purified anti-Bcl-XS/L Antibody or 1:5000 diluted Direct-Blot™ HRP anti-GAPDH Antibody, clone W17079A (lower panel). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Check Availability Save
633901 25 µg $101
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633902 100 µg $218
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Description

Bcl-X belongs to Bcl-2 family, and has several alternative splicing isoforms. It plays a critical role in control of apoptosis. The large isoform Bcl-XL (26kD) contains the highly conserved BH1/BH2 domain and protects cells from apoptosis, whereas the short isoform, Bcl-XS (19kD), which lacks of conserved BH1/BH2 domain, promotes apoptosis by interacting with Bcl-XL and Bcl-2. Bcl-XS is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-XL is found in long-lived, post-mitotic cells, such as adult brain.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Bcl-XS/L (3-14 amino acid, conserved in both human and mouse)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted at between 2°C and 8°C.
Application

WB - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
Product Citations
  1. Kim HY, et al. 2014. Nat Med. 54:20. PubMed
  2. Hollenbaugh JA, et al. 2016. Virology. 106:491. PubMed
  3. Wang F, et al. 2020. Int J Oncol. 57:707. PubMed
  4. Inao T, et al. 2018. Oncotarget. 144:318. PubMed
RRID
AB_2064706 (BioLegend Cat. No. 633901)
AB_2064706 (BioLegend Cat. No. 633902)

Antigen Details

Structure
Five alternative splicing isoforms: Bcl-XS, Bcl-XL, Bcl-Xβ, Bcl-X, Bcl-XδTM
Distribution

Bcl-XS is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-XL is found in long-lived, post-mitotic cells, such as adult brain.

Function
Bcl-X plays a critical role in the control of apoptosis. The large isoform Bcl-XL (26kD) contains the highly conserved BH1/BH2 domain and protects cells from apoptosis, whereas the short isoform, Bcl-XS (19kD), which lacks of conserved BH1/BH2 domain, promotes apoptosis by interacting with Bcl-XL and Bcl-2.
Interaction
Bcl-2, Cytochrome C, JNK1, BAG 1, Caspase 8, Caspase 9, c-FLIP, Bax, p53
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Boise LH, et al. 1993 Cell 74:597

Gene ID
598 View all products for this Gene ID
UniProt
View information about Bcl-XS/L on UniProt.org
Go To Top Version: 2    Revision Date: 08/21/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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