- Clone
- 16C12B70 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CCAAT/enhancer-binding protein alpha, C/EBP-A
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
662102 | 100 µg | $265 |
The CCAAT-enhancer binding proteins (C/EBP) are members of the basic region/leucine zipper (bZIP) transcription factors, that bind to the consensus CCAAT sequence in DNA. C/EBPα is expressed in two isoforms: p42 and p30 by ultilizing different translational start codons. The C/EBPα p30 isoform has little to no transactivating activity and acts as an inhibitor. C/EBPα regulates expression of various myeloid-specific genes and is required for myeloid differentiation. C/EBPα has been found to play a key role in adipogenesis, since inhibition of C/EBPα blocks adipocyte differentiation. C/EBPα also regulates cell cycle progression through interactions with cyclin-dependent kinases (CDK) and by inducing the expression of CDK inhibitor p21. Mutations in the C/EBPα gene are associated with the development of acute myeloid leukemia.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide (AKQLALAGLFPYQPPPP) conjugated to KLH.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product weakly reacts with Mouse.
- Product Citations
-
- RRID
-
AB_2564224 (BioLegend Cat. No. 662102)
Antigen Details
- Structure
- 358 amino acids with a predicted molecular weight of 38 kD; contains a bZIP domain responsible for DNA binding.
- Distribution
-
Nucleus
- Function
- C/EBPα is a bZIP transcription factor that regulates proliferation and differentiation of myeloid cells and adipocytes.
- Interaction
- C/EBPα forms heterodimers with C/EBPβ and γ; interacts with CDK2, CDK4, PRDM16, UBN1, and ZNF638.
- Cell Type
- Neutrophils
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. De Obaldia ME, et al. 2013. Nat. Immunol. 14:1277.
2. Di Tullio A, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:17016.
3. Fan HY, et al. 2011. Mol. Endocrinol. 25:253.
4. Cai DH, et al. 2008. Oncogene. 27:2772.
5. Khanna-Gupta A. 2008. Blood Cells Mol. Dis. 41:77.
6. Janardhan HP. 2008. Sci. Signal. 1:jc2. - Gene ID
- 1050 View all products for this Gene ID
- UniProt
- View information about C/EBPalpha on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All C/EBPα Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-C/EBPα | 16C12B70 | WB,ICC |
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