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Purified anti-CaMKII Antibody

Pricing & Availability
Clone
6G9 (See other available formats)
Regulatory Status
RUO
Other Names
CAMK2, CaM kinase II, Calcium/calmodulin-dependent protein kinase type II subunit alpha
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6G9_PURE_CaMKII_Antibody_1_WB_121619_updated.png
Whole cell extracts (15 µg protein) from the indicated tissue lysates were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CaMKII antibody (clone 6G9) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 6G9_PURE_CaMKII_Antibody_1_WB_121619_updated.png
    Whole cell extracts (15 µg protein) from the indicated tissue lysates were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CaMKII antibody (clone 6G9) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 6G9_PURE_CaMKII_Antibody_2_IF_062716
    C57BL/6 mouse frozen brain section was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes then blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of anti-CaMKII (clone 6G9) antibody and 1:1000 anti-GFAP (clone Poly28400) antibody overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG and Alexa Fluor® 488 (green) conjugated goat anti-mouse IgG for one hour at room temperature. The nuclei were
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688602 100 µg $340
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Description

The Ca2+/calmodulin (CaM)-dependent protein kinases (CaMKs) are multifunctional serine/threonine kinases whose activity are regulated through Ca2+ signaling. CaMKII is expressed as a multimeric protein, which typically is comprised of 12 subunits in physiological conditions. CaMKII is an important member of the calcium/calmodulin-activated protein kinase family. It phosphorylates nearly 40 different proteins, including enzymes, ion channels, kinases, and transcription factors. CaMKII plays critical roles in neural synaptic stimulation and T cell receptor signaling. Recent studies suggested that high levels of CaMKII is also expressed in variety of cancers. It regulates proliferation, differentiation and survival of cancer cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
50% pure type II CaM kinase, purified through Calmodulin-Sepharose step.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. For immunohistochemical staining on frozen tisse sections, a concentration range of 2.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Erondu NE, et al. 1985. J. Neurosci. 5:3270.
RRID
AB_2617027 (BioLegend Cat. No. 688602)

Antigen Details

Structure
478 amino acids with a predicted molecular weight of 50 kD.
Distribution

Synapse and cytosol.

Function
A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release; member of the NMDAR signaling complex in excitatory synapses it regulates NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Interaction
BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1.
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase, TCRs
Antigen References

1. Hook SS, et al. 2001. Annu. Rev. Pharmacol. Toxicol. 41:471.
2. Hoelz A, et al. 2003. Mol. Cell 11:1241.
3. Lisman J, et al. 2012. Nat. Rev. Neurosci. 13:169.
4. Barria A, et al. 1997. Science 276:2042.
5. Hughes K, et al. 2001. J. Biol. Chem. 276:36008.
6. Wang YY, et al. 2015. Oncotarget. 6:11725.

Gene ID
815 View all products for this Gene ID
UniProt
View information about CaMKII on UniProt.org
Go To Top Version: 2    Revision Date: 07/01/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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