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Purified anti-CASK Antibody (Previously Covance catalog# MMS-5128)

Pricing & Availability
Clone
K56A/50 (See other available formats)
Regulatory Status
RUO
Other Names
Peripheral plasma membrane protein CASK, LIN2
Previously
Covance Catalog# MMS-5128
Isotype
Mouse IgG1, κ
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Product Citations
publications
K56A50_Pure_CASK_Antibody_1_021318
Western blot of purified anti-CASK antibody (clone K56A/50). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • K56A50_Pure_CASK_Antibody_1_021318
    Western blot of purified anti-CASK antibody (clone K56A/50). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • K56A50_Pure_CASK_Antibody_2_021318
    IHC staining of purified anti-CASK antibody (clone K56A/50) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • K56A50_Pure_CASK_Antibody_3_021318
    IHC staining of purified anti-CASK antibody (clone K56A/50) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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830601 100 µL $165
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Description

Calcium/Calmodulin-Dependent Serine Protein Kinase (CASK) is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. It contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. It binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 318-415 of rat CASK.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. For immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsueh YP, et al. 1999. J Neurosci. 19:7415.
RRID
AB_2564940 (BioLegend Cat. No. 830601)

Antigen Details

Structure
Expected MW: 100 kD
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
29647 View all products for this Gene ID
UniProt
View information about CASK on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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