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Purified anti-Cbl Antibody

Pricing & Availability
Clone
Poly6330 (See other available formats)
Regulatory Status
RUO
Other Names
E3 ubiquitin-protein ligase Cbl, Proto-oncogene c-Cbl
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly6330_PURE_Cbl_WB_040418
Total cell lysates (15 µg protein) from HepG2 (Lane 1), HeLa (Lane 2), Raji (Lane 3), HEL (Lane 4), or RAW264.7 (Lane 5) cells were resolved using 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-Cbl antibody (Poly6330) at a 1:1000 dilution. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit-IgG (Cat. No. 406401) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) at a 1:5000 dilution (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing CBL mRNA expression levels (source: Human Protein Atlas).
  • Poly6330_PURE_Cbl_WB_040418
    Total cell lysates (15 µg protein) from HepG2 (Lane 1), HeLa (Lane 2), Raji (Lane 3), HEL (Lane 4), or RAW264.7 (Lane 5) cells were resolved using 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-Cbl antibody (Poly6330) at a 1:1000 dilution. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit-IgG (Cat. No. 406401) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) at a 1:5000 dilution (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing CBL mRNA expression levels (source: Human Protein Atlas).
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633001 125 µL $112
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Description

The proto-oncogene c-Cbl was initially identified as the cellular homologue of v-Cbl oncogene that induces pre-B cell lymphomas and myeloid leukemias in mice. The Cbl family comprises three mammalian proteins, c-Cbl, Cbl-b, and Cbl-3; among these, c-Cbl and Cbl-b are expressed in hemopoietic cells, whereas Cbl-3 is expressed only in epithelial tissues. Cbl proteins can function as E3 ubiquitin (Ub) ligases, and in more recent studies Cbl has been implicated in the negative regulation of various RTKs.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
peptide mapping to the carboxy terminal domain of human Cbl
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.  Western blotting suggested working dilution(s): Use 5 µl per 5 ml antibody (1:1000) dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This polyclonal was generated using an immunogen that corresponds to the C-terminus of c-Cbl. Due to poor sequence homology between the C-termini of c-Cbl and other Cbl family members, we do not predict this antibody will cross-react with other Cbl proteins.

At concentrations greater than a 1:1000 dilution, this antibody may produce minor non-specific bands in certain lysates.

Additional Product Notes
The 125 µL size can be used for approximately 25 Western blots.
RRID
SCR_001134 (BioLegend Cat. No. 633001)

Antigen Details

Structure
c-Cbl is a 906 amino acid protein with a predicted molecular weight of 99.6 kDa. The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. C-terminal contains proline-rich domain with potential tyrosine phosphorylation sites.
Distribution

Ubiquitous cytoplasmic protein

Interaction
NCK, CD2AP, SLA, SLA2, EGFR, SYK, LAT2 and ZAP70
Bioactivity
E3 ubiquitin (Ub) ligases, and negative regulators of PTKs
Biology Area
Cell Biology, Ubiquitin/Protein Degradation
Antigen References

1. Liu YC, et al. 1998. Cell Signal 10:377.

Gene ID
867 View all products for this Gene ID
UniProt
View information about Cbl on UniProt.org
Go To Top Version: 2    Revision Date: 04/04/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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