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Purified anti-CCL7 (MCP-3) Antibody

Pricing & Availability
Clone
A15027D (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte chemotactic protein3 (MCP-3), Small inducible cytokine subfamily A, member 7 (SCYA7)
Isotype
Mouse IgG1, κ
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Product Citations
publications
A15027D_PURE_CCL7_Antibody_WB_050616
Recombinant human CCL7 (50 ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-CCL7 (MCP-3) (clone A15027D) antibody. Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection.
  • A15027D_PURE_CCL7_Antibody_WB_050616
    Recombinant human CCL7 (50 ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-CCL7 (MCP-3) (clone A15027D) antibody. Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection.
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686702 100 µg $340
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Description

CCL7, also known as MCP-3, was initially purified from cytokine-stimulated human osteosarcoma cell line (MG-63) conditioned medium. CCL7 has high similarity with CCL2/MCP-1 (71%), CCL8/MCP-2 (58%), and CCL13/MCP-4 (59%) at the amino acid level. Chemokines play a key role in inflammation and, in the initial phase, CXC chemokines chemoattract polymorphonuclear leukocytes (PMNs). The next step in the inflammatory processes is directed in part by CC chemokines, including the monocyte chemoattractant proteins (CCL2, CCL7, CCL8, and CCL13) that chemoattract additional leukocytes subsets. It has been reported that matrix metalloproteinases (MMPs) regulate chemokine bioactivity, increasing or decreasing their chemoattractant property. MMP-8 and MMP-9 modulate CXC chemokines, and MMP-2 truncates the N-terminal of CCL7, eliminating the first four amino acids producing CCL7 (5-76 residues), which leads to a loss of activity. The truncated CCL7 binds to its receptor, but it does not induce signaling transduction. Monocyte chemoattractant proteins (MCPs) are truncated by different MMPs to create potent antagonists and induce decreases in inflammation. For example, MMP-13 cleaves CCL2 and CCL7 and generates potent receptor antagonists. Also, CCL7 and CCL2 play an important role in the pathogenesis of systemic sclerosis (SSc). It has been described that they are overexpressed in SSc and activate collagen production in dermal fibroblasts. In addition, CCL7 and CCL21 are highly expressed in gastric cancer and are associated with lymph node metastasis and poor prognosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human CCL7.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2616886 (BioLegend Cat. No. 686702)

Antigen Details

Structure
Chemokine.
Distribution
Fibroblasts, mast cells, astrocytes, endothelial cells, monocytes, and macrophages.
Function
CCL7 chemoattracts monocytes, T cells, NK cells, eosinophils, and basophils. CCL7 protein is secreted by mononuclear leukocytes after stimulation with IL-1β, IFN-α, IFN-β, measles virus, poly I-C, or Con A. In fibroblasts, CCL7 mRNA and protein are synergistically induced after treatment with IL-1β and IFN-γ. CCL7 is stimulated in murine fibroblasts and astrocytes by TGF-β and TNF-α respectively. Anti-inflammatory cytokines (IL-4, IL-10, or IL-13) inhibit the expression of CCL7 in monocytes.
Interaction
Monocytes, T cells, NK cells, dendritic cells, mast cells, eosinophils, and basophils.
Ligand/Receptor
CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195).
Cell Type
Fibroblasts, Mast cells, Astrocytes, Endothelial cells, Monocytes, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Van Damme J, et al. 1992. J. Exp. Med. 176:59.
2. Menten P, et al. 1999. Eur. J. Immunol. 29:678.
3. McQuibban GA, et al. 2000. Science 289:1202.
4. Menten P, et al. 2001. Eur. Cytokine Netw. 12:554.
5. Dean RA, et al. 2008. Blood 112:3455.
6. Renner NA, et al. 2011. J. Neurovirol. 17:146.
7. Hwang TL, et al. 2012. World J. Gastroenterol. 18:1249.
8. Feuser K, et al. 2012. Cytokine 58:178.

Gene ID
6354 View all products for this Gene ID
UniProt
View information about CCL7 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/13/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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