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Purified anti-CD248 Antibody

Pricing & Availability
Clone
VI-71 (See other available formats)
Regulatory Status
RUO
Other Names
TEM1, Endosialin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
VI-71_PURE_CD248_Antibody_030422
IMR-32 cells were fixed with ice-cold methanol for 10 minutes and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:100 dilution) of purified mouse IgG1, κ isotype control (Cat. No. 400102) (panel A) or 2.0 µg/mL purified anti-CD248 (clone VI-71) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.5 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
  • VI-71_PURE_CD248_Antibody_030422
    IMR-32 cells were fixed with ice-cold methanol for 10 minutes and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:100 dilution) of purified mouse IgG1, κ isotype control (Cat. No. 400102) (panel A) or 2.0 µg/mL purified anti-CD248 (clone VI-71) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.5 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
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949901 25 µg $118
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949902 100 µg $293
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Description

CD248, or TEM1, is classified as a C-type lectin-like transmembrane glycoprotein localized in the plasma membrane. CD248 is expressed mainly during embryonic development. In normal human adults, its expression is retained in very low levels in endometrial stroma, bone marrow fibroblasts, and corpus luteum. In adults with cancer, CD248 can be found to be expressed in tumor stroma and endothelium. Fibronectin and collagen I/IV have been reported to be ligands of CD248. Through this interaction, tumor cells overexpressing CD248 have increased cellular migration and adhesive properties, which in turn may support tumor progression and invasion. Mac-2 BP/90K has also been identified as a CD248 ligand and data suggests that CD248-Mac2 BP/90K interactions may also play an important role during tumor progression and metastasis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide for human CD248
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not suitable for WB.

This clone does not stain formalin-fixed paraffin- embedded tissue by IHC-IF.

Product Citations
  1. Lu T, et al. 2023. Cancer Immunol Immunother. :. PubMed
RRID
AB_2910519 (BioLegend Cat. No. 949901)
AB_2910519 (BioLegend Cat. No. 949902)

Antigen Details

Structure
CD248 is a 757 amino acid protein with a predicted molecular weight of 81 kD.
Distribution

Plasma membrane

Function
Embryonic development
Ligand/Receptor
Fibronectin; collagen I/IV; Mac-2 BP/90K
Biology Area
Cell Adhesion, Cell Death, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability
Antigen References
  1. Becker R, et al. 2008. FASEB J. 22:3059-67.
  2. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569-75.
  3. Teicher B. 2019. Oncotarget. 10:993-1009.
  4. Tomkowicz B. et al. 2007. Proc Natl Acad Sci U S A. 104:17965-70.
Gene ID
57124 View all products for this Gene ID
UniProt
View information about CD248 on UniProt.org
Go To Top Version: 1    Revision Date: 03/04/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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