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Purified anti-CD340 (ErbB2/HER-2) Antibody

Pricing & Availability
Clone
W22266D (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine-protein kinase ErbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W22266D_PURE_CD340_WB_100324
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-CD340 (ErbB2/HER-2) (clone W22266D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22266D_PURE_CD340_WB_100324
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-CD340 (ErbB2/HER-2) (clone W22266D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22266D_PURE_CD340_IHC-P_2_100324
    IHC staining of Purified anti-CD340 (ErbB2/HER-2) (clone W22266D) on formalin-fixed paraffin-embedded human breast cancer tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 925501), the tissue was incubated without (panels A and C) and with (panels B and D) Purified anti-CD340 (ErbB2/HER-2) (clone W22266D), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 465416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panels A and B). Images were captured with a 40X objective. Scale bar: 50 µm
  • C. W22266D_PURE_CD340_ICC_100324
    SK-BR-3 cells were fixed with ice-cold methanol and then stained with Purified anti-CD340 (ErbB2/HER-2) (clone W22266D) (panels B and D) or Purified rat IgG2a, κ isotype control (Cat. No. 400501) (panels A and C) followed by Alexa Fluor® 647 Goat anti-Rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801) (Panels C and D). The images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar 50 µm
  • D. W22266D_PURE_CD340_ICFC_100324
    HeLa cells (negative control, open histogram), or SK-BR-3 cells (positive control, filled histogram) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Purified anti-CD340 (ErbB2/HER-2) (clone W22266D) or Purified rat IgG2a, κ isotype control (Cat. No. 400501) (open histogram, dashed line) (representative histogram for both cell lines) followed by Alexa Fluor™ 647 Goat anti-rat IgG (Cat. No. 405416).
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639951 25 µg $180
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639952 100 µg $455
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Description

CD340, also commonly known as HER-2 or ErbB2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two CheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand- binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in lung adenocarcinoma, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. CD340 is a therapeutic target in several human cancers and it has been shown that overexpression in breast cancers confers Taxol resistance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the intracellular domain of human CD340 (ErbB2/HER-2)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody (clone W22266D) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol, or fixation/permeabilization with 100% ice-cold methanol alone.

For use of this antibody (clone W22266D) in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Fixation/permeabilization using fixation buffer (Cat. No. 420801) followed by True-Phos™ Perm Buffer (Cat. No. 425401) is not recommended.

For use of this antibody (clone W22266D) in immunohistochemistry (IHC), we recommend antigen retrieval with either Sodium Citrate H.I.E.R. (Cat. No. 928502) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703).

This antibody (clone W22266D) does not cross-react with mouse CD340 (ErbB2/HER2) in Western Blot.

Antigen Details

Structure
CD340 is a 1,255 amino acid protein with a predicted molecular weight of 137.9 kD. It is a glycosylated receptor-like transmembrane protein; due to glycosylation, the observed molecular weight by Western Blot is closer to 175-185 kD.
Distribution

Cellular membrane and also present in internalized vesicles

Function
Receptor, transcriptional regulator
Interaction
Homodimerizes
Heterodimerizes with EGFR, ErbB3, ErbB4, PIK3C2B, KPNB1, RANBP2, EEA1, CRM1, CLTC, MUC1, PTK6, RPA194, ACTB, PRKCABP, SRC, MYOC, CPNE3, SORL1, SH3BGRL
Ligand/Receptor
Heterodimerizes with other ERBB family members upon ligand binding, but does not directly bind ligand
Cell Type
Leukemia
Biology Area
Cell Biology, Immunology, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Akiyama T, et al. 1986. Science. 232:1644-6.
  2. Bargmann CI, et al. 1986. Nature. 319:226-30.
  3. Pegram MD, et al. 1997. Oncogene. 15:537-47.
  4. Slamon DJ, et al. 1989. Science. 244:707-12.
  5. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID
2064 View all products for this Gene ID
UniProt
View information about CD340 on UniProt.org
Go To Top Version: 1    Revision Date: 10/03/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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