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Purified anti-CDC2 Phospho (Tyr15) Antibody

Pricing & Availability
Clone
A21009D (See other available formats)
Regulatory Status
RUO
Other Names
Cyclin Dependent Kinase 1, Cdk1, CDC28A, P34CDC2, P34 Protein Kinase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a. A21009D_PURE_CDC2_WB_032423
Whole cell extracts (15 µg total protein) from HeLa cells treated with 4 mM hydroxyurea for 20 hours (+) and untreated HeLa cells (-) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-CDC2 Phospho (Tyr15) (clone A21009D) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using a pan CDC2 (clone POH-1) (Cat. No. 626901) and Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. A21009D_PURE_CDC2_WB_032423
    Whole cell extracts (15 µg total protein) from HeLa cells treated with 4 mM hydroxyurea for 20 hours (+) and untreated HeLa cells (-) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-CDC2 Phospho (Tyr15) (clone A21009D) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using a pan CDC2 (clone POH-1) (Cat. No. 626901) and Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. A21009D_PURE_CDC2_-ICFC-_032423
    HeLa cells treated with hydroxyurea (4 mM, 20 hours) (positive control, filled histogram) and untreated HeLa cells (negative control, open histogram) were fixed with ice-cold methanol and permeabilized using the True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with purified anti-CDC2 Phospho (Tyr15) (clone A21009D) or purified Mouse IgG1, κ isotype control (Cat. No. 400102) (open histogram, dashed line) followed by PE goat anti-mouse IgG (Cat. No. 405307).
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613851 25 µg $125
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613852 100 µg $335
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Description

Cell division control protein 2 (CDC2) also known as Cyclin-dependent kinase 1 (CDK1) is a key regulatory kinase essential for cell growth, proliferation, cell cycle progression, and early embryonic development. It is responsible for driving cells through G2 phase into M phase. Prior to mitosis, CDC2, along with its binding partner cyclin B1, exists in an inactive state when phosphorylated on Thr14 and Tyr15 by PKMYT1 (MYT1) and WEE1. This B1-CDC2 complex is activated during G2 and early mitosis by CDC2-mediated dephosphorylation. During mitosis, CDC2 inhibits DNA re-replication and subsequent polyploidy and cell senescence through the sequestering of cyclin A2 and suppression of the formation of CDK2-cyclin A complexes. CDC2 can function as a substitute for other CDC family members and drive the progression of the cell cycle in the event of their loss. CDC2 functions as a positive regulator of global translation, protein synthesis, and genome stability and participates in DNA damage repair, checkpoint activation, and DNA replication fork progression. It is overexpressed in several cancers including liver, breast, and colorectal cancers and has been implicated in the promotion of tumorigenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human CDC2 phosphorylated at tyrosine 15
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2941613 (BioLegend Cat. No. 613851)
AB_2941613 (BioLegend Cat. No. 613852)

Antigen Details

Structure
CDC2 is a 297 amino acid protein with a predicted molecular weight of 34 kD.
Distribution

Ubiquitously expressed/ Mitochondria, nucleus, cytoskeleton, cytoplasm

Function
Serine/threonine-protein kinase/ Cell Cycle
Biology Area
Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication
Molecular Family
Phospho-Proteins
Antigen References
  1. Chow J, et al. 2010. Mol Cell Biol. 31:1478-91.
  2. Diril M, et al. 2012. PNAS. 109:3826-31.
  3. Haneke K, et al. 2020. J Cell Biol. 219:e201906147.
  4. Liao H, et al. 2017. Oncotarget. 8:90662-90673. 
  5. Prevo R, et al. 2018. Cell Cycle. 17:1513-1523.

 

Gene ID
983 View all products for this Gene ID
UniProt
View information about CDC2 on UniProt.org
Go To Top Version: 1    Revision Date: 03/24/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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