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Purified anti-CDC20 Antibody

Pricing & Availability
Clone
W18156A (See other available formats)
Regulatory Status
RUO
Other Names
Cell Division Cycle 20, Cell Division Cycle Protein 20 Homolog, BA276H19.3, CDC20A, BA276H19.3, p55CDC
Isotype
Rat IgG2a, κ
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Product Citations
publications
W18156A_PURE_CDC20_Antibody_1_111119.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-CDC20 antibody (clone W18156A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18156A_PURE_CDC20_Antibody_1_111119.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-CDC20 antibody (clone W18156A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18156A_PURE_CDC20_Antibody_2_111419_updated.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502) or purified anti-CDC20 antibody (clone W18156A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CDC20 antibody (clone W18156A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP anti-mouse IgG, light chain specific antibody. Lane M: Molecular weight marker.
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934701 25 µg $101
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934702 100 µg $253
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Description

CDC20 is a co-activator of the anaphase-promoting complex or cyclosome (APC/C) and is a cell cycle regulator that initiates the metaphase-to-anaphase transition. In early mitosis, APC/C is phosphorylated by Cdk1 and CDC20 binds to the APC/C. CDC20 activates the APC/C-mediated ubiquitination of anaphase inhibitors, including securin and cyclin B. Degradation of these proteins results in the activation of separase, which cleaves the Scc1 subunit of cohesion complex to trigger chromosome segregation, and thereby promoting mitotic exit.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human CDC20 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize mouse CDC20.

This clone recognized a ~75 kD protein of unknown origin by western blot and immunoprecipitation. Because of this, we have not tested or recommend this clone for immunocytochemistry.

Due to the presence of the ~75 kD protein of unknown origin in W18156A immunoprecipitates, we do not recommend the clone to be used for co-immunoprecipitation applications.

RRID
AB_2820217 (BioLegend Cat. No. 934701)
AB_2820217 (BioLegend Cat. No. 934702)

Antigen Details

Structure
CDC20 is a 499 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

Centrosome/ubiquitously expressed

Function
Coactivator/Cell cycle regulator
Biology Area
Cell Cycle/DNA Replication
Antigen References
  1. Visintin R, et al. 1997. Science. 278: 460.
  2. Fang G, et al. 1998. Mol. Cell. 2:163.
  3. Shirayama M, et al. 1999. Nature. 402:203.
  4. Yu H. 2007. Mol. Cell. 27:3.
  5. Pesin JA and Orr-Weaver TL. 2008. Annu. Rev. Cell. Dev. Biol. 24:475.
  6. Izawa D and Pines J. 2011. Nat Cell Biol. 13:223
Gene ID
991 View all products for this Gene ID
UniProt
View information about CDC20 on UniProt.org
Go To Top Version: 1    Revision Date: 11/11/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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