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Purified anti-CDK4 Antibody

Pricing & Availability
Clone
W15200A (See other available formats)
Regulatory Status
RUO
Other Names
Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W15200A_PURE_CDK4_Antibody_WB_090616
Total cell lysate from human HeLa cells (lane 1), 293T cells (lane 2), and NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-CDK4 (clone W15200A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
  • W15200A_PURE_CDK4_Antibody_WB_090616
    Total cell lysate from human HeLa cells (lane 1), 293T cells (lane 2), and NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-CDK4 (clone W15200A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
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690802 100 µg $253
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Description

Cyclin-Dependent Kinase 4 (CDK4) is a member of the Ser/Thr protein kinase family. It functions as the catalytic subunit of the cyclin D-CDK4 (DC) complex that is important for cell cycle progression through the G1 phase. The DC complexes accumulate on the nuclear membrane and are transported to the nucleus when cells enter S phase. In the nucleus, DC complexes phosphorylate and inhibit the retinoblastoma (Rb) proteins. Phosphorylation of Rb protein results in dissociation of the transcription factor E2F from the Rb/E2F complex and activation of E2F target genes. This process is controlled by D-type cyclins and CDK inhibitor p16 (CDK4I).  Formation of the ternary complex, cyclin D/CDK4/CDKN1B, is required for nuclear translocation and DC complex activity. Mutations of CDK4 gene have been found to be associated with the tumorigenesis of many cancers such as cutaneous malignant melanoma.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length human CDK4 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629825 (BioLegend Cat. No. 690802)

Antigen Details

Structure
303 amino acids with a predicted molecular weight of 33.7 kD. Contains a Ser/Thr protein kinase domain.
Distribution
Cytoplasm when non-clomplexed, nucleus, and nuclear membrane.
Function
CDK4 is a Ser/Thr kinase and is a component of D-CDK4 complex that regulates cell cycle progression during G1/S transition.
Interaction
Forms a complex with the D-type G1 cyclins CND1, CCND2, and CCND3. Interacts with SEI1, ZNF655, CDKN1A, CDNN1B, CDKN2A, and CEBPA.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. O'Leary B, et al. 2016. Nat. Rev. Clin. Oncol. 13:417.
2. Jirawatnotai S, et al. 2014. Cell Cycle 13:2889.
3. Lee Y, et al. 2014. Nature 510:547.
4. Sheppard KE, McArthur GA. 2013. Clin. Cancer Res. 19:5320.
5. Wang C, et al. 2011. Cell Cycle 10:57.
6. Paternot S. 2010. Cell Cycle 9:689.

Gene ID
1019 View all products for this Gene ID
UniProt
View information about CDK4 on UniProt.org
Go To Top Version: 1    Revision Date: 09/09/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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