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Purified anti-COMT Antibody

Pricing & Availability
Clone
A17180E (See other available formats)
Regulatory Status
RUO
Other Names
Catechol-O-methyltransferase (COMT), Epididymis Secretory Sperm Binding Protein Li 98n, Testicular Tissue Protein Li 42, EC 2.1.1.6, HEL-S-98n
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_A17180E_PURE_COMT_Antibody_4_031722
IHC staining using purified anti-COMT (clone A17180E) on formalin-fixed paraffin-embedded human placenta tissue. The tissue was incubated with 5 μg/mL of anti-COMT antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (red) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scalebar = 50 μM
  • 1_A17180E_PURE_COMT_Antibody_4_031722
    IHC staining using purified anti-COMT (clone A17180E) on formalin-fixed paraffin-embedded human placenta tissue. The tissue was incubated with 5 μg/mL of anti-COMT antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (red) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scalebar = 50 μM
  • 2_A17180E_PURE_COMT_Antibody_1_031722
    HeLa cells were fixed with 100% ice-cold methanol for 10 minutes and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with either 5.0 µg/mL (1:100 dilution) purified mouse IgG1, κ isotype control (Cat. No. 401402) (panel A) or 1.25 µg/mL purified anti-COMT (clone A17180E) (panel B) overnight at 4 °C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 1:200 dilution. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale bar = 50 μm
  • 3_A17180E_PURE_COMT_Antibody_2_031722
    NTERA2 Cells (low expressing target) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with 0.03 µg of purified anti-COMT (clone A17180E) (filled histogram) or 0.03 µg/test of purified mouse IgG1, κ isotype control (Cat. No. 400102) (open histogram) followed by PE goat anti-mouse IgG (Cat. No. 405307).
  • 4_A17180E_PURE_COMT_Antibody_3_031722
    HeLa cells (high expressing target) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with 0.03 µg of purified anti-COMT (clone A17180E) (filled histogram) or 0.03 µg/test of purified mouse IgG1, κ isotype control (Cat. No. 400102) (open histogram) followed by PE goat anti-mouse IgG (Cat. No. 405307).
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604551 25 µg $118
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604552 100 µg $335
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Description

Catechol-O-methyltransferase (COMT) catalyzes the addition of methyl groups to catecholamine hormones and neurotransmitters; in particular: dopamine, norepinephrine, and epinephrine. Norepinephrine and epinephrine are key mediators of the sympathetic nervous system, while dopamine is an important neurotransmitter in the regulation of mood and behavior. Since O-methylation inactivates target catecholamines and leads to its degradation, COMT activity has significant implications for human health. The Val158Met polymorphism of the COMT gene is associated with mental disorders such as schizophrenia, obsessive compulsive disorder, bipolar disorder, and substance dependence.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human COMT
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 2.5 μg/mL is recommended. For intracellular flow cytometric staining, the suggested concentration range of this reagent is 0.01 - 0.03 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Product is not recommended for Western Blot. 

For ICC, recommend fixation/permeabilization with 100% ice-cold methanol. Do not recommend fixation with 4% PFA.

RRID
AB_2922640 (BioLegend Cat. No. 604551)
AB_2922640 (BioLegend Cat. No. 604552)

Antigen Details

Structure
COMT is a 271 amino acid protein with a predicted molecular weight of 30.0 kD.
Distribution

COMT can be found in the cytosol and on the plasma membrane.

Function
Methyltransferase
Cell Type
Dopaminergic Neurons, Mature Neurons, Neurons
Biology Area
Neurodegeneration, Neuroscience, Neuroscience Cell Markers
Molecular Family
Enzymes and Regulators
Antigen References
  1. Akhtar MJ, et al. 2020. Bioorg Chem. 94:103418.
  2. Scheggia D, et al. 2012. CNS Neurol Disord Drug Targets. 11:209-21.
  3. Hosak L. 2007. Eur Psychiatry. 22:276-81.
Gene ID
1312 View all products for this Gene ID
UniProt
View information about COMT on UniProt.org
Go To Top Version: 1    Revision Date: 03/17/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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