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Purified anti-CRY2 Antibody

Pricing & Availability
Clone
W17237A (See other available formats)
Regulatory Status
RUO
Other Names
Cryptochrome Circadian Regulator 2, Cryptochrome 2 (Photolyase-Like), Cryptochrome Circadian Clock 2, Cryptochrome-2, Growth-Inhibiting Protein 37, KIAA0658, HCRY2, PHLL2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17237A_PURE_CRY2_Antibody_1_030320
Whole cell extracts (15 µg protein) from NIH/3T3 cells transfected with non-targeting control siRNA (siCON) or siRNA targeting CRY2 (siCRY2) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CRY2 antibody (clone W17237A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W17237A_PURE_CRY2_Antibody_1_030320
    Whole cell extracts (15 µg protein) from NIH/3T3 cells transfected with non-targeting control siRNA (siCON) or siRNA targeting CRY2 (siCRY2) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CRY2 antibody (clone W17237A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W17237A_PURE_CRY2_Antibody_2_030320
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CRY2 antibody (clone W17237A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Cell lysates were loaded in descending order of predicted CRY2 expression; predicted expression data was obtained from Human Protein Atlas. Lane M: Molecular weight marker. *: Protein of unknown origin.
  • W17237A_PURE_CRY2_Antibody_3_030320
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CRY2 antibody (clone W17237A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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936301 25 µg $101
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936302 100 µg $253
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Description

Cryptochrome 2 (CRY2) regulates core clock genes and participates in the maintenance of circadian rhythms in mammalian cells. CRY2 modulates a transcriptional autoregulatory feedback loop in circadian clocks. Two core clock proteins, CLOCK and BMAL1 forms a heterodimer, which binds to the promoter region of CRY and Periods (PER) genes to activate the expression of these genes. CRY2 forms a heterodimer with PER, translocates to the nucleus, and represses the CLOCK/BMAL1, thereby regulating circadian rhythms.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CRY2 partial recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes both human and mouse CRY2.

When tested for western blot, this clone recognized a ~30 kD band of unidentified origin in human cell lines.

This clone failed to immunoprecipitate CRY2 during product development testing and is not recommended for the application.

When tested for ICC, this clone failed to stain RAW264.7 cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol; we therefore do not recommend it for the application.

RRID
AB_2832910 (BioLegend Cat. No. 936301)
AB_2832910 (BioLegend Cat. No. 936302)

Antigen Details

Structure
CRY2 is a 593 amino acid protein with a predicted molecular weight of 70 kD
Distribution

Nucleus

Function
Circardian Rhythm
Biology Area
Cell Biology, Transcription Factors
Antigen References
  1. van der Horst GT, et al. 1999. Nature. 398:627.
  2. Yu W, et al. 2002. Biochem Biophys Res Commun. 290:933.
  3. Huber AL, et al. 2016. Mol Cell. 64:774.
  4. Chiou YY, et al. 2016. Proc Natl Acad Sci U S A. 113:E6072.
Gene ID
1408 View all products for this Gene ID
UniProt
View information about CRY2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/03/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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