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Purified anti-Cyclin D2 Antibody

Pricing & Availability
Clone
W15213A (See other available formats)
Regulatory Status
RUO
Other Names
CCND2, G1/S-specific cyclin-D2
Isotype
Rat IgG2a, κ
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Product Citations
publications
W15213A_PURE_CyclinD2_Antibody_WB_011217
Total cell lysates (15 µg protein) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-Cyclin D2 (clone W15213A) antibody and competitor’s clone at the concentration suggested by the manufacturer. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (clone Poly4053) or secondary antibody appropriate to competitors clone, and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. For additional WB data, see supplemental data.
  • W15213A_PURE_CyclinD2_Antibody_WB_011217
    Total cell lysates (15 µg protein) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-Cyclin D2 (clone W15213A) antibody and competitor’s clone at the concentration suggested by the manufacturer. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (clone Poly4053) or secondary antibody appropriate to competitors clone, and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. For additional WB data, see supplemental data.
See additional WB data...
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693702 100 µg $265
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Description

D-type cyclins (D1, D2, D3) are mainly involved in regulation of transition from G1 to S phase during the cell cycle. These cyclins share the capacity to activate both CDK4 and CDK6, which leads to the phosphorylation of the retinoblastoma tumor suppressor protein (RB). The phosphorylation of RB consequently activate transcription factors such as E2F, which enhances the activation of transcription of genes involved in DNA synthesis and cells transition to S phase. The dysregulation of these proteins disturbs cell cycle and promotes the carcinogenesis.

Cyclin D2 is a member of the D-type cyclin family and mainly mediates the extracellular signaling environment with cell cycle progression. Overexpression of Cyclin D2 has been reported in testicular germ cell tumor cell lines, colon cancer and gastric cancer. Paradoxically, D-type cyclins were also shown to inhibit cell growth by inducing a senescence-like phenotype and inhibiting cell proliferation. Several studies showed reduced level of cyclin D2 in breast, lung, prostate, renal, pancreatic and gastric cancer.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length of human cyclin D2 expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W15213A does not cross-react with mouse (in-house tested).

This clone recognizes both isoforms of Cyclin D2.

This clone was not evaluated by immunofluorescence or immunoprecipitation.

RRID
AB_2650677 (BioLegend Cat. No. 693702)

Antigen Details

Structure
Two isoforms with a predicted molecular weight of 33 kD and 24.5 kD.
Distribution

Nucleus, cytoplasm, membrane.

Function
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition.
Interaction
CDK4, CDK5, CDK6.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Gene ID
894 View all products for this Gene ID
UniProt
View information about Cyclin D2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/16/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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