Loading...

Purified anti-Cytokeratin, HMW Antibody

Pricing & Availability
Clone
34βE12 (See other available formats)
Regulatory Status
RUO
Other Names
Cytokeratin, high-molecular weight keratin (HMWK), high-molecular weight cytokeratin (HMWCK)
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
34betaE12_PURE_Cytokeratin_HMW_IHCP_071216
IHC staining of anti-Cytokeratin, HMW antibody (clone 34βE12) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody at 0.7 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • 34betaE12_PURE_Cytokeratin_HMW_IHCP_071216
    IHC staining of anti-Cytokeratin, HMW antibody (clone 34βE12) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody at 0.7 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
See high resolution IHC-P data...
Cat # Size Price Quantity Check Availability Save
916504 100 µg $229
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

This antibody is specific for high molecular weight cytokeratins 1, 5, 10 and 14. Clone 34βE12 is immunoreactive with squamous and adeno-squamous carcinomas. In normal epithelia, the antibody stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This antibody is also useful in differentiating benign and malignant tumors of the prostate gland.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using a solubilized keratin extracted from human stratum corneum.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/ml is suggested. For Western blotting, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The Cytokeratin, High Molecular Weight (HMW) antibody (clone 34ßE12) reacts primarily with complex epithelium such as squamous, ductal and transitional epithelium and corresponding tumors.

Application References

(PubMed link indicates BioLegend citation)
  1. Gown AM, Vogel AM. 1985. Am. J. Clin. Pathol. 84:413. (IHC)
  2. Gown AM, Vogel AM. 1982. J. Cell Biol. 95:414. (IHC)
RRID
AB_2629548 (BioLegend Cat. No. 916504)

Antigen Details

Distribution

Tissue distribution: skin, prostate or squamous cell carcinoma.
Cellular distribution: cytoplasm.

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Cytokeratin on UniProt.org
Go To Top Version: 1    Revision Date: 07/20/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account