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Purified anti-DNM1L Antibody

Pricing & Availability
Clone
W20081E (See other available formats)
Regulatory Status
RUO
Other Names
Dynamin-1-like protein (DNM1L), Dnm1p/Vps1p-like protein (DVLP), Dynamin family member proline-rich carboxyl-terminal domain less (DYMPLE), Dynamin-like protein 1 (DLP1), Dynamin-related protein 1 (DRP1)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20081E_PURE_DNM1L_Antibody_032822
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-DNM1L (clone W20081E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • W20081E_PURE_DNM1L_Antibody_032822
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-DNM1L (clone W20081E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • W20081E_PURE_DNM1L_Antibody_2_032922
    ICC staining of purified anti-DNM1L (clone W20081E) on HeLa cells. The cells were fixed and permeabilized with ice cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 2.5 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 60X objective.
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603851 25 µg $118
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603852 100 µg $293
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Description

Dynamin-1-like protein (DNM1L) is a member of the dynamin superfamily of GTPases. Its best studied role is in the control of mitochondrial fission. DNM1L oligomerizes into filaments that form rings around the scission site to “pinch off” dividing mitochondria using GTP hydrolysis. Accordingly, DNM1L also has important functions in the mitochondrial dynamics of cell proliferation and apoptosis. DNM1L is also required for proper formation of the nervous system, and deficiencies in its functions have been linked to Alzheimer’s Disease. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human DNM1L protein fragments
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 2.5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For Western Blot, higher molecular weight band (≈100 kDa) likely corresponds to SUMOylated DNM1L (e.g. Figueroa-Romer, et al. 2009). 

For ICC, we recommend fixation/permeabilization with 100% ice cold methanol alone. Alternatively, 4% paraformaldehyde fixation followed by permeabilization with ice cold methanol is possible. We do not recommend 4% paraformaldehyde fixation followed by permeabilization with 0.5% Triton-X, due to incorrect signal localization. 

Not recommended for use in IHC-P (human). 

RRID
AB_2922636 (BioLegend Cat. No. 603851)
AB_2922636 (BioLegend Cat. No. 603852)

Antigen Details

Structure
DNM1L is a 736 amino acid protein with a predicted molecular weight of 81.8 kD.
Distribution

DNM1L is widely expressed in the cytoplasm, mitochondria, and peroxisomes of most tissues. 

Function
DNM1L is a GTPase
Interaction
CDK1
Biology Area
Cell Motility/Cytoskeleton/Structure, Mitochondrial Function
Molecular Family
Cytoskeletal Proteins, GTPases
Antigen References
  1. Oliver D and Reddy PH. 2019. Cells. 8:961. 
  2. Reddy PH, et al. 2011. Brain Res Rev. 67:103-18. 
Gene ID
10059 View all products for this Gene ID
UniProt
View information about DNM1L on UniProt.org
Go To Top Version: 1    Revision Date: 03/28/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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