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Purified anti-E. coli RNA Sigma 54 Antibody

Pricing & Availability
Clone
6RN3 (See other available formats)
Regulatory Status
RUO
Other Names
rpoN, glnF, ntrA, RNA Polymerase
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
6RN3_PURE_E-coli_RNA_Sigma_54_Antibody_101019.png
Whole cell extracts (15 µg protein) from DH5α E. coli cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-E. coli RNA Sigma 54 Antibody, clone 6RN3, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution.
  • 6RN3_PURE_E-coli_RNA_Sigma_54_Antibody_101019.png
    Whole cell extracts (15 µg protein) from DH5α E. coli cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-E. coli RNA Sigma 54 Antibody, clone 6RN3, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution.
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663305 25 µg $101
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663306 100 µg $253
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Description

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and then are released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (Sigma 54 and core RNA polymerase) serves as the receptor for the receipt of the melting signal from the remotely bound activator protein glnG (ntrC).

Product Details
Technical data sheet

Product Details

Verified Reactivity
E. coli
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Previously item# W0005 from Neoclone.

Clone 6RN3 recognizes epitope 88-137.

Additional reported applications (for the relevant formats) include: functional assay.1

Application References

(PubMed link indicates BioLegend citation)
  1. Zhao K, et al. 2010. Nucleic Acids Res. 38:1273. (FA)
RRID
AB_2820109 (BioLegend Cat. No. 663305)
AB_2820109 (BioLegend Cat. No. 663306)

Antigen Details

Structure
Sigma 54 (σ54) is a 54 kD transcription factor of E. coli RNA polymerase. Observed molecular weight is around 70 kD.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947714 View all products for this Gene ID
UniProt
View information about Sigma 54 on UniProt.org
Go To Top Version: 3    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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