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Purified anti-EGFR Phospho (Tyr845) Recombinant Antibody

Pricing & Availability
Clone
QA19A93 (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A93_PURE_EGFR_Phospho_Tyr845_Antibody_020221
Whole cell extracts (15 µg protein) from untreated (-) and recombinant human EGF-treated (+) (Cat. No. 585506) A-431 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-EGFR Phospho (Tyr845) recombinant antibody (clone QA19A93) for two hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Total EGFR loading was confirmed using purified anti-EGFR antibody (clone A19002A) (Cat. No. 933902) at 0.05 µg/mL. Lane M: Molecular weight marker.
  • QA19A93_PURE_EGFR_Phospho_Tyr845_Antibody_020221
    Whole cell extracts (15 µg protein) from untreated (-) and recombinant human EGF-treated (+) (Cat. No. 585506) A-431 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-EGFR Phospho (Tyr845) recombinant antibody (clone QA19A93) for two hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Total EGFR loading was confirmed using purified anti-EGFR antibody (clone A19002A) (Cat. No. 933902) at 0.05 µg/mL. Lane M: Molecular weight marker.
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943401 25 µg $153
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943402 100 µg $347
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Description

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the protein. Phosphorylation at tyrosine 845, which is found in the kinase activation loop, stabilizes the active conformation and leads to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human EGFR phosphorylated at tyrosine 845
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC on A-431 cells treated with or without EGF. We failed to detect any EGFR Phospho (Tyr845) staining using the following fixation and permeabilization methods:

  • 4% PFA plus Triton X-100
  • 4% PFA plus methanol
  • Methanol only
This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells.

This recombinant antibody clone is derived from clone A18028A.

This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete sequence conservation with the immunizing peptide.
RRID
AB_2890866 (BioLegend Cat. No. 943401)
AB_2890866 (BioLegend Cat. No. 943402)

Antigen Details

Structure
EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution

Plasma membrane/Ubiquitously expressed

Function
EGFR signaling, cell signaling
Ligand/Receptor
EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF
Cell Type
Epithelial cells
Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
1. Hunter T and Cooper JA. 1981. Cell. 24:741.
2. Gill GN and Lazar CS. 1981. Nature. 293:305.
3. Reynolds FH, et al. 1981. Nature. 292:259
4. Biscardi, et al. 1999. J. Biol. Chem. 274:8335.
5. Zhou M, et al. 2013. Cancer Res. 23:7056-67
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org
Go To Top Version: 2    Revision Date: 03/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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