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Purified anti-eIF4A1 Antibody

Pricing & Availability
Clone
W17130C (See other available formats)
Regulatory Status
RUO
Other Names
EIF4A1, Eukaryotic Translation Initiation Factor 4A1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17130C_Pure_eIF4A1_Antibody_1_102618
Western blot analysis of 15 µg cell lysates from HL-60, HaCaT, HepG2, HeLa (Human), Raw264.7 (Mouse), UMR106 (Rat) and CHO (Hamster) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-EIF4A1 antibody, clone W17130C. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 664804, 1:25000 dilution).
  • W17130C_Pure_eIF4A1_Antibody_1_102618
    Western blot analysis of 15 µg cell lysates from HL-60, HaCaT, HepG2, HeLa (Human), Raw264.7 (Mouse), UMR106 (Rat) and CHO (Hamster) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-EIF4A1 antibody, clone W17130C. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 664804, 1:25000 dilution).
  • W17130C_Pure_eIF4A1_Antibody_2_103118_updated
    Immunoprecipitation analysis using purified anti-eIF4A1 antibody (Clone W17130C). 150 or 300 µg of HeLa cell lysates was incubated with 10 µg (20 µL) eIF4A1 overnight, 10 µg Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502) was incubated with 300 µg HeLa cell lysates as negative control. 15 µg (10% of IP) cell lysates was used as input.
  • W17130C_Pure_eIF4A1_Antibody_3_102618
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Purified Rat IgG2a, κ Isotype Ctrl Antibody (Negative, Cat. No. 400502) or (B-D) Purified anti-EIF4A1 antibody (clone W17130C) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/30. Concentrations for (A-B) is 8 µg/mL, (C) is 2 µg/mL, (D) is 0.5 µg/mL and (E) is 0.1 µg/mL.
  • W17130C_Pure_eIF4A1_Antibody_4_103118_updated
    Immunoprecipitation analysis using purified anti-eIF4A1 antibody (Clone W17130C). 150 or 300 µg of HeLa cell lysates was incubated with 10 µg (20 µL) eIF4A1 overnight, 10 µg Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502) was incubated with 300 µg HeLa cell lysates as negative control. 15 µg (10% of IP) cell lysates was used as input.
  • W17130C_Pure_eIF4A1_Antibody_5_102618
    IHC staining of purified anti-eIF4A1 antibody (clone W17130C) on formalin-fixed paraffin-embedded (FFPE) human (A, C) and mouse (B, D) colon tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with (A, B) or without (C, D) 5 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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666201 25 µg $101
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666202 100 µg $253
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Description

EIF4A1 is an ATP-dependent RNA helicase. It can form the eIF4F complex, which is involved in translation initiation by binding with the 5' m7 GTP cap of the mRNA. It could unwind mRNA secondary structure at the 5’ UTR region and lead to the small ribosomal subunit binding and followed scanning of the initiator codon.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, Hamster
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human EIF4A1 Peptide (3-19 a.a.) conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 µg/mL (1:1000 - 1:20000 dilution). For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/mL (1:250 - 1:1000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 15 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/mL (1:50 - 1:100 dilution) is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783367 (BioLegend Cat. No. 666201)
AB_2783367 (BioLegend Cat. No. 666202)

Antigen Details

Distribution

Cytoplasm

Function
Translation initiation
Interaction
eIF4 complex
Biology Area
Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Synthesis, Signal Transduction
Gene ID
1973 View all products for this Gene ID
UniProt
View information about eIF4A1 on UniProt.org
Go To Top Version: 1    Revision Date: 10/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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