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Purified anti-eIF4G1 Antibody

Pricing & Availability
Clone
W18079D (See other available formats)
Regulatory Status
RUO
Other Names
Eukaryotic Translation Initiation Factor 4 Gamma 1, EIF-4-Gamma 1, EIF-4-Gamma 1, EIF-4G1, EIF4GI, EIF4F, EIF4G, p220, PARK18
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18079D_PURE_eIF4G1_Antibody_1_091619.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-eIF4G1 Antibody, clone W18079D, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W18079D_PURE_eIF4G1_Antibody_1_091619.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-eIF4G1 Antibody, clone W18079D, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W18079D_PURE_eIF4G1_Antibody_2_091619.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:500 dilution (1.0 µg/mL) of either Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502, panel A) or Purified anti-eIF4G1 Antibody, clone W18079D (Panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG Antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • W18079D_PURE_eIF4G1_Antibody_3_091619.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502) or Purified anti-eIF4G1 Antibody, clone W18079D. The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of eIF4G1. Lane M: Molecular Weight marker.
Cat # Size Price Quantity Check Availability Save
933301 25 µg $101
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933302 100 µg $253
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Description

Eukaryotic initiation factor 4 gamma 1 (eIF4G1), the most abundant isoform of the eIF4G family, is an important component of the eIF4F complex, which plays a role in cap-dependent protein synthesis. eIF4G1 is a scaffolding protein that provides a platform for other translation initiation factors, including eIF4E, eIF4A, and the eIF3 complex. Protein synthesis is promoted in the presence of growth factors and hormones through the stimulation of tyrosine kinase receptors, which in turn activate the mTOR pathway. mTOR phosphorylates 4EBP1, which leads to the binding of eIF4E to eIF4G1 and thereby facilitates the assembly of the eIF4F complex. In addition to the initiation factors, eIF4G1 interacts with poly-A-binding protein (PABP), which promotes the circularization of mRNA by bridging the 5’ cap to the 3’ poly-A tail.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human eIF4G1 protein (amino acids 101-350)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes both human and mouse eIF4G1.

Both Triton X-100 and methanol permeabilization produced strong and specific signal in ICC testing.

RRID
AB_2814641 (BioLegend Cat. No. 933301)
AB_2814641 (BioLegend Cat. No. 933302)

Antigen Details

Structure
eIF4G1 is a 1599 amino acid protein with a predicted molecular weight of 175 kD.
Distribution

Cytosol/Ubiquitously expressed

Function
Translation
Biology Area
Protein Synthesis
Molecular Family
Scaffold Proteins
Antigen References
  1. Hentze M. 1997. Science. 275:500.
  2. Tarun S, et al. 1997. Proc Natl Acad Sci USA. 94:9046.
  3. Gross JD, et al. 2003. Cell. 115:739-50.
  4. Hinton T, et al. 2007. J. Biol. Chem. 282:1695.
Gene ID
1981 View all products for this Gene ID
UniProt
View information about eIF4G1 on UniProt.org
Go To Top Version: 1    Revision Date: 09/16/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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