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Purified anti-E. coli CRP Antibody

Pricing & Availability
Clone
1D8D9 (See other available formats)
Regulatory Status
RUO
Other Names
cAMP receptor protein, cAMP regulatory protein, Catabolite gene activator, cap, csm
Isotype
Mouse IgG1
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Product Citations
publications
1D8D9_Pureaf_EcoliCRP_Antibody_WB_030515
Western blot analysis of 50 ng recombinant CRP protein (lane 1), E. coli (BL21) (lane 2), E. coli (DH5α) (lane 3), and E. coli (Rosetta) (lane 4). Anti- E. coli CRP antibody (clone 1D8D9 ) was used, followed by HRP anti-mouse IgG antibody.
  • 1D8D9_Pureaf_EcoliCRP_Antibody_WB_030515
    Western blot analysis of 50 ng recombinant CRP protein (lane 1), E. coli (BL21) (lane 2), E. coli (DH5α) (lane 3), and E. coli (Rosetta) (lane 4). Anti- E. coli CRP antibody (clone 1D8D9 ) was used, followed by HRP anti-mouse IgG antibody.
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664304 100 µg $323
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Description

CRP (also known as cAMP receptor protein) is a regulatory protein in bacteria. CRP binding to cAMP leads to conformational changes in CRP resulting in a dramatic increase in its binding to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA and acts as a negative regulator of its own synthesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
E. coli
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified protein
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Huang Y, et al. 2022. Gut Microbes. 14:2136460. PubMed
  2. Gibson JA, et al. 2022. Proc Natl Acad Sci U S A. 119:e2210115119. PubMed
  3. Lauritsen I, et al. 2021. Nat Commun. 12:5880. PubMed
  4. Ayhan D, et al. 2016. PLoS Biol. 14: 1002552. PubMed
  5. Seok JY, et al. 2021. Cell Reports. 36(8):109589. PubMed
  6. Pryor R et al. 2019. Cell. 178(6):1299-1312 . PubMed
RRID
AB_2565553 (BioLegend Cat. No. 664304)

Antigen Details

Structure
210 amino acids with a predicted molecular weight of approximately 23.5 kD.
Distribution

Bacteria.

Function
Transcriptional regulator in bacteria.
Ligand/Receptor
cAMP
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Busby S, et al. 1999. J. Mol. Biol. 293:199.
2. Lawson CL, et al. 2004. Curr. Opin. Struct. Biol. 14:10.
3. Hudson BP, et al. 2009. Proc. Natl. Acad. Sci. USA 106:19830.

Gene ID
1036667 View all products for this Gene ID
UniProt
View information about CRP on UniProt.org

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Go To Top Version: 2    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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