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Purified anti-FAF1 Antibody

Pricing & Availability
Clone
CPTC-FAF1-1 (See other available formats)
Regulatory Status
RUO
Other Names
FAS-associated factor 1, hFAF1, UBX domain-containing protein 12, UBX domain-containing protein 3A, UBXD12, UBXN3A, Fas (TNFRSF6) Associated Factor 1, TNFRSF6-Associated Factor 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
CPTC-FAF1-1_PURE_FAF1_Antibody_1_120219
Western blot of purified anti-FAF1 antibody (clone CPTC-FAF1-1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of human glioblastoma brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • CPTC-FAF1-1_PURE_FAF1_Antibody_1_120219
    Western blot of purified anti-FAF1 antibody (clone CPTC-FAF1-1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of human glioblastoma brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • CPTC-FAF1-1_PURE_FAF1_Antibody_2_120219
    Western blot of purified anti-FAF1 antibody (clone CPTC-FAF1-1). Lane 1: Molecular weight marker; Lane 2: 20 µg of HeLa cell lysate; Lane 3: 20 µg of A431 cell lysate; Lane 4: 20 µg of U251 cell lysate (negative control). The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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870601 25 µg $106
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870602 100 µg $276
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Description

FAS-associated factor 1 (FAF1) is an evolutionarily conserved protein with multiple protein interaction domains, including a FAS-interacting domain (FID), a death effector domain interacting domain (DEDID), and multi-ubiquitin-related domains. Initially identified as a member of FAS death-inducing signaling complex, FAF1 plays diverse roles in apoptotic and survival pathways. It may also serve as a scaffolding protein that regulates protein degradation in the ubiquitin-proteasome pathway. FAF1 is abundantly expressed in the brain where it modulates cell death. FAF1 also serves as a substrate for parkin, a key protein implicated in the pathogenesis of Parkinson’s disease (PD). Wild type parkin ubiquitinates FAF1 and promotes its degradation, while PD-linked mutant parkin causes abnormal accumulation of FAF1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820196 (BioLegend Cat. No. 870601)
AB_2820196 (BioLegend Cat. No. 870602)

Antigen Details

Structure
Human FAF1 is a 650 amino acid protein with a predicted molecular mass of 74 kD.
Distribution

Tissue distribution: Ubiquitously expressed
Cellular distribution: Plasma membrane, nucleus, cytosol, endoplasmic reticulum

Function
FAF1 is an ubiquitin-binding protein, required for the progression of DNA replication forks.
Interaction
Interacts with CDT1, ATPase VCP/p97, FAS, and NLRP12.
Cell Type
Neurons
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Cell Death, Neuroscience, Ubiquitin/Protein Degradation
Antigen References
  1. Menges CW, et al. 2009. Cell Cycle. 8(16):2528-34.
  2. Sul JW, et al. 2013. Hum Mol Genet. 22(8):1558-73.
  3. Cheng K, et al. 2011. J Neurosci. 31(33):11905-13.
Gene ID
11124 View all products for this Gene ID
UniProt
View information about FAF1 on UniProt.org
Go To Top Version: 1    Revision Date: 12/02/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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