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Purified anti-FGF-19 Antibody

Pricing & Availability
Clone
13D9C80 (See other available formats)
Regulatory Status
RUO
Other Names
Fibroblast growth factor 19
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
13D9C80_PURE_FGF-19_Antibody_WB_100115
20 or 50 ng of human FGF-19 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-19 (clone 13D9C80) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 13D9C80_PURE_FGF-19_Antibody_WB_100115
    20 or 50 ng of human FGF-19 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-19 (clone 13D9C80) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
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681102 100 µg $288
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Description

FGF-19 belongs to a subfamily of the fibroblast growth factors (FGFs) called endocrine FGFs, which consists of FGF-19, FGF-21, and FGF-23. Unlike other subfamily of FGFs, FGF-19 has a low binding affinity to heparin sulfate and functions as a circulating hormone with effects on distant tissues. FGF-19 has reduced binding affinity to the FGF receptors and the interaction requires two necessary cofactors that are α-Klotho and β-Klotho. In the ileum, FGF-19 expression is initiated by bile acids-induced activation of farnesoid X receptor (FXR). In response to bile acid stimulation, FGF-19 is secreted and enters the portal venous circulation, which subsequently down-regulates hepatic bile acid synthesis through repression of the CYP7A1 gene encoding cholesterol 7-alpha-monooxygenase. The impaired regulation of bile acids by FGF-19 leads to bile acid diarrhea also known as idiopathic bile acid malabsorption. FGF-19 has also been demonstrated to regulate carbohydrate and lipid metabolism.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human FGF-19 recombinant protein (25-216 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566443 (BioLegend Cat. No. 681102)

Antigen Details

Structure
Growth factor. 216 amino acids with a predicted molecular weight of 24 kD.
Distribution
Secreted.
Function
FGF-19 functions as a hormone, regulating homeostasis of bile acid, glucose, lipids, phosphate, and vitamin D.
Interaction
Interacts with heparian, α-Klotho, and β-Klotho.
Ligand/Receptor
FGFR1, FGFR2, FGFR3, and FGFR4.
Biology Area
Cell Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Xie MH, et al. 1999. Cytokine 11:729.
2. Harmer NJ, et al. 2004. Biochemistry 43:629.
3. Goetz R, et al. 2007. Mol. Cell Biol. 27:3417.
4. Wu X, et al. 2008. J. Biol. Chem. 283:33304.
5. Song KH, et al. 2009. Hepatology 49:297.
6. Turner N, et al. 2010. Nat. Rev. Cancer 10:116.

Gene ID
9965 View all products for this Gene ID
UniProt
View information about FGF-19 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/01/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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