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Purified anti-FGF-3 Antibody

Pricing & Availability
Clone
W15075A (See other available formats)
Regulatory Status
RUO
Other Names
INT2, INT-2, Heparin-binding growth factor 3, HNGF-3, HNGF3, FGF3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W15075A_PURE_FGF3_Antibody_041216
20 or 50 ng of human FGF-3 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-3 (clone W15075A) antibody. Proteins were visualized using an HRP Goat anti-rat IgG antibody and chemiluminescence detection.
  • W15075A_PURE_FGF3_Antibody_041216
    20 or 50 ng of human FGF-3 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-3 (clone W15075A) antibody. Proteins were visualized using an HRP Goat anti-rat IgG antibody and chemiluminescence detection.
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685102 100 µg $241
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Description

FGF-3 is a member of the fibroblast growth factor family. The amplification of the FGF-3 gene has been found in many cancer types including squamous cell carcinoma, epithelial ovarian tumors, breast cancer and bladder cancer. FGF-3 is also associated with tumor metastasis and recurrence in human hepatocellular carcinoma. Like many other FGF proteins, FGF-3 plays an important role in embryonic development. FGF-3 is needed for normal inner ear development including placode induction, maintenance, and otic vesicle formation. The role of FGF-3 in ear development is conserved amongst different vertebrates including mouse, chicken, and zebrafish. In addition to FGF-3, it has been shown that FGF-8 and FGF-10 play redundant and unique roles in ear development as well. FGF-3 is also important for early hindbrain patterning. FGF-3 is derived from hindbrain boundary cells and regulates the expression of multiple markers at hindbrain boundaries. It has been reported that FGF-3 is involved in cardiovascular development in two forms. These two forms of FGF-3 are a secreted form that induces proliferation and another form that localizes in the nucleus and inhibits cell proliferation. The nuclear isoform binds rpS2 and this binding has been suggested to interfere with ribosomal biogenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human FGF-3 recombinant protein (28-212 a.a.) expressed in E.coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2616756 (BioLegend Cat. No. 685102)

Antigen Details

Structure
192 amino acids with a predicted molecular weight of 22 kD. Belongs to the fibroblast growth factor family.
Distribution
Secreted.
Function
FGF-3 plays important roles in embryonic development, cell proliferation, and differentiation. Retinoic acid regulates the signal transduction of FGF-3 in the inner ear development.
Interaction
Ribosomal protein S2.
Ligand/Receptor
FGF-3 binds to the IIIb isoforms of FGFR1 and FGFR2 with high affinity and binds to the IIIc isoforms of FGFR2 with low affinity.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Kiefer P, et al. 1991. Mol. Cell Biol. 11:5929.
2. Brookes S, et al. 1989. Oncogene 4:429.
3. Zelarayan LC, et al. 2007. Dev. Biol. 308:379.
4. Fekete DM. 2000. Trends Neurosci. 23:332.
5. Leger S, Brand M. 2002. Mech. Dev. 119:91.
6. Weisinger K, et al. 2012. Biol. Open 1:67.
7. Hatch EP, et al. 2007. Development 134:3615.
8. Antoine M, et al. 2005. Biochem. Biophys. Res. Commun. 338:1248.
9. Frenz DA, et al. 2010. Am. J. Med. Genet. A. 152A:2947.

Gene ID
2248 View all products for this Gene ID
UniProt
View information about FGF-3 on UniProt.org
Go To Top Version: 1    Revision Date: 04/14/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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