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Purified anti-FIG4/SAC3 Antibody

Pricing & Availability
Clone
N202/7 (See other available formats)
Regulatory Status
RUO
Other Names
SAC3, SAC domain-containing protein 3, Polyphosphoinositide phosphatase, Phosphatidylinositol 3,5-bisphosphate 5-phosphatase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N202slash7_Purified_FIG4-SAC3_Antibody_032519.png
Western blot of purified anti-FIG4 antibody (clone N202/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 436303) was used as the detection system.
  • N202slash7_Purified_FIG4-SAC3_Antibody_032519.png
    Western blot of purified anti-FIG4 antibody (clone N202/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 436303) was used as the detection system.
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861801 25 µg $83
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861802 100 µg $165
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Description

Phosphoinositides (PIs) are signaling molecules that regulate vesicle trafficking in eukaryotic cells and are important for maintaining the nervous system. The levels of PIs are regulated by specific protein kinases and phosphatases. FIG4 protein (the homolog of yeast SAC3 protein), is a phosphatase well conserved from mammals to yeast, and is known to dephosphorylate phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) at the 5th position of inositol. By interacting with FAB1 and VAC14, the protein complex regulates the overall level of PI(3,5)P2. Loss of function of FIG4 leads to the reduction of the cellular PI(3,5)P2 levels. Mutations of human FIG4 gene cause autosomal recessive Charcot-Marie-Tooth type 4BJ (CMT4BJ) neuropathy with hereditary motor and sensory degeneration. Variants of FIG4 gene have also been found in patients with amyotrophic lateral sclerosis (ALS) and primary lateral sclerosis (PLS), suggesting it may be a risk factor for these disorders.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein amino corresponding to amino acids 688-907 (C-terminus) of mouse FIG4.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801233 (BioLegend Cat. No. 861801)
AB_2801233 (BioLegend Cat. No. 861802)

Antigen Details

Structure
Human FIG4 is a 907 amino acid protein with a molecular mass of 103 kD.
Distribution

Tissue distribution: ubiquitously expressed.
Cellular source: plasma membrane, endoplasmic reticulim, endosome, golgi apparatus, intracellular membrane, and vacuoles.

Function
FIG4 is involved in the regulation of phosphoinositides levels and intracellular vesicle trafficking.
Interaction
Interacts with FAB1 and VAC14
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family
Protein Kinases/Phosphatase, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Chow C. et al. 2007. Nature. 448(7149):68-72.
  2. Chow C. et al. 2009. Am J Hum Genet. 84(1):85-8.
  3. Lenk G.M. et al. 2011. PLoS Genet. 7(6):e1002104.
Gene ID
9896 View all products for this Gene ID 103199 View all products for this Gene ID 309855 View all products for this Gene ID
UniProt
View information about FIG4/SAC3 on UniProt.org
Go To Top Version: 1    Revision Date: 03/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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