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Purified anti-FITC Antibody

Pricing & Availability
Clone
FIT-22 (See other available formats)
Regulatory Status
RUO
Other Names
Fluorescein isothiocyanate
Isotype
Mouse IgG1, λ
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Product Citations
publications
A_FIT-22_Pure_111607
C57BL/6 thymocytes stained with RM4-5 FITC only (top) or RM4-5 FITC plus purified FIT-22 (bottom), then detected with anti-mouse IgG PE
  • A_FIT-22_Pure_111607
    C57BL/6 thymocytes stained with RM4-5 FITC only (top) or RM4-5 FITC plus purified FIT-22 (bottom), then detected with anti-mouse IgG PE
  • B_FIT-22_Pure_111607
    C57BL/6 thymocytes stained with RM4-5 FITC only (top) or RM4-5 FITC plus purified FIT-22 (bottom), then detected with anti-mouse IgG PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
408301 50 µg $83
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408302 500 µg $293
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Description

The FIT-22 antibody reacts with both fluorescein isothiocyanate (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FITC conjugated mouse IgG.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. 

Product Citations
  1. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  2. Hattori Y, et al. 2023. Cell Rep. 42:112092. PubMed
  3. Yang LF, et al. 2022. Anal Chem. 94:12683. PubMed
  4. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  5. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  6. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
RRID
AB_528900 (BioLegend Cat. No. 408301)
AB_528900 (BioLegend Cat. No. 408302)

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Go To Top Version: 3    Revision Date: 10/18/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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