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Purified anti-FOXO1 Antibody

Pricing & Availability
Clone
2F8B08 (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein O1A (FOXO1A), Forkhead in rhabdomyosarcoma (FKHR)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2F8B08_Purified_FOXO1_Antibody_1_092418
Total cell lysates (15 µg total protein) from HEL (negative control, Lane 1), HeLa (reduced expression control, Lane 2), and Daudi cells (Lane 3) were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-FOXO1 Antibody, clone 2F8B08, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 2F8B08_Purified_FOXO1_Antibody_1_092418
    Total cell lysates (15 µg total protein) from HEL (negative control, Lane 1), HeLa (reduced expression control, Lane 2), and Daudi cells (Lane 3) were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-FOXO1 Antibody, clone 2F8B08, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 2F8B08_Purified_FOXO1_Antibody_2_092418
    293T cells were stained with purified anti-FOXO1 (clone 2F8B08) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
  • 2F8B08_Purified_FOXO1_Antibody_3_092418
    Immunoprecipitation of FOXO1 from Hela cell extracts. Lane 1 is 8% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-FOXO1 antibody (clone 2F8B08, lane 4). Western blot was performed using anti-FOXO1 antibody (clone 2F8B08).
  • 4. 2F8B08_PURE_FoxO_Antibody_06102022
    IHC staining using purified anti-FOXO1 (clone 2F8B08) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated with 10 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 μM
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658102 100 µg $241
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Description

FOXO1 is a transcription factor, belonging to the O subfamily of forkhead box-containing proteins. The forkhead proteins are substrates of Akt/PKB and glucocorticoid-induced kinases that mediate insulin signaling. FOXO1 binds to the conserved insulin-responsive elements and acts as a transcriptional activator. The FOXO1 target genes are involved in cell metabolism, growth, and differentiation. Upon insulin stimulation, FOXO1 proteins are phosphorylated by AKT/PKB through the PI3K pathway, resulting in the translocation of FOXO1 proteins from the nucleus to the cytoplasm. The expression of FOXO1 target genes are then inhibited by FOXO1. FOXO1 has been reported to regulate maturation and class-switch recombination of B cells, as well as participating in regulatory T cell function. FOXO1-deficient mice are embryonic lethal, suggesting that FOXO1 has crucial functions in development which cannot be compensated by other FoxO family members.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human FOXO1 recombinant protein (544-655aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 8.0 - 10. µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2562793 (BioLegend Cat. No. 658102)

Antigen Details

Structure
655 amino acids, predicted molecular weight of 70 kD. Contains a C-terminal trans-activating domain and a Fork-head domain responsible for DNA binding.
Distribution

Cytoplasm, Nucleus

Function
FOXO1 is a transcription factor mediating Insulin signaling. It regulates expression of the genes involved in cell growth, differentiation, and metabolism.
Interaction
FOXO1 interacts with RUNX2, NLK, SIRT1, PRMT1, SKP2, ATF4, and p300.
Cell Type
B cells, Tregs
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Trinh DL, et al. 2013. Blood. 121:3666.
2. Ouyang W, et al. 2012. Nature. 491:554. 
3. Ferdous A, et al. 2012. Transcription. 3:221.
4. Cifarelli V, et al. 2012. Mol. Endocrinol. 26:1213.
5. Kamagate A, et al. 2008. Cell Cycle. 7:3162.
6. Kousteni S. 2012. Bone. 50:437.

Gene ID
2308 View all products for this Gene ID
UniProt
View information about FOXO1 on UniProt.org
Go To Top Version: 7    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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