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Purified anti-Fumarase Antibody

Pricing & Availability
Clone
9B1-4E1-8D5 (See other available formats)
Regulatory Status
RUO
Other Names
Fumarate Hydratase, FH, MCL, HLRCC, MCUL1, FMRD
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. 9B1-4E1-8D5_PURE_Fumarase_WB_062223
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL purified anti-Fumarase (clone 9B1-4E1-8D5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:10000 dilution. Lane M: Molecular weight marker
  • A. 9B1-4E1-8D5_PURE_Fumarase_WB_062223
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL purified anti-Fumarase (clone 9B1-4E1-8D5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:10000 dilution. Lane M: Molecular weight marker
  • B. 9B1-4E1-8D5_PURE_Fumarase_IHCP_062223
    IHC staining of purified anti-Fumarase (clone 9B1-4E1-8D5) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Tris-EDTA pH 9.0 (10 mM Tris, 1 mM EDTA), the tissue was incubated with 5.0 µg/mL of purified anti-Fumarase (clone 9B1-4E1-8D5) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with 10X (panel A) and 40X (panel B) objectives. Scale bar: 50 µm
  • C. 9B1-4E1-8D5_PURE_Fumarase_ICC_062223
    HeLa cells were co-stained with 100 nM of MitoSpy™ Red CMXRos (Cat. No. 424801) (panel A) for 20 minutes at 37°C, fixed and permeabilized with ice-cold methanol for 10 minutes, then blocked with 5% FBS for 1 hour at room temperature. Cells were then stained overnight with 2.5 µg/mL of purified anti-Fumarase (clone 9B1-4E1-8D5), followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) (panel B) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel C). Merged images show colocalization in mitochondria (panel D). Images were captured with a 63X objective. Scale bar: 20 µm
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616851 25 µg $167
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616852 100 µg $416
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Description

Fumarase, or fumarate hydratase, is a Krebs cycle enzyme that catalyzes the conversion of fumarate to L-malate. A cytosolic isoform of fumarase has been described that is required for arginine metabolism and the urea cycle. Inactivating germline mutations of the FH gene are found in patients with hereditary leiomyomatosis and renal cell cancer syndrome, which predisposes patients to increased risk of developing uterine, cutaneous leiomyomas, and renal cell carcinomas. Mechanistically, elevated fumarate functions as an oncometabolite by inhibiting the activity of α-ketoglutarate dependent enzymes, such as hypoxia-inducible factor prolyl hydroxylases (PHDs) and ten-eleven translocation (TET) dioxygenases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human fumarase
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product does not recognize mouse and rat Fumarase. For cross-reactivity to mouse and rat, refer to clone W18319C (Cat. No. 940001). 

For immunocytochemistry, ice-cold methanol-only fixation is recommended.

RRID
AB_3068159 (BioLegend Cat. No. 616851)
AB_3068159 (BioLegend Cat. No. 616852)

Antigen Details

Structure
Fumarase is a 510 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

Ubiquitously expressed/Mitochondrial, Cytosolic

Function
Catalyzes fumarate to L-malate
Interaction
H2AZ1
Biology Area
Cell Biology, Mitochondrial Function
Molecular Family
Enzymes and Regulators
Antigen References
  1. Laukka T, et al. 2016. J Biol Chem. 291:4256-65.
  2. Sciavoceli M, et al. 2016. Nature. 537:544-547.
Gene ID
2271 View all products for this Gene ID
UniProt
View information about Fumarase on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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