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Purified anti-Galectin-7 Antibody

Pricing & Availability
Clone
A18059A (See other available formats)
Regulatory Status
RUO
Other Names
Galectin 7, Gal-7, GAL7, HKL-14, PI7, PIG1, p53-induced gene 1 protein, TP53I1, LGALS7A
Isotype
Rat IgG2a, κ
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Product Citations
publications
A18059A_PURE_Galectin-7_Antibody_1_120219
Western blot of purified anti-Galectin-7 antibody (clone A18059A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human skin lysate; Lane 3: 20 µg of mouse skin lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-rat IgG antibody (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system. The upper band detected in human skin lysate is the dimer form of Galectin-7.
  • A18059A_PURE_Galectin-7_Antibody_1_120219
    Western blot of purified anti-Galectin-7 antibody (clone A18059A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human skin lysate; Lane 3: 20 µg of mouse skin lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-rat IgG antibody (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system. The upper band detected in human skin lysate is the dimer form of Galectin-7.
  • A18059A_PURE_Galectin-7_Antibody_2_120219
    IHC staining of purified anti-Galectin-7 antibody (clone A18059A) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A18059A_PURE_Galectin-7_Antibody_3_120219
    IHC staining of purified anti-Galectin-7 antibody (clone A18059A) on formalin-fixed paraffin-embedded mouse skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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866301 25 µg $106
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866302 100 µg $276
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Description

The galectins are a family of beta-galactoside-binding proteins implicated in cell-cell and cell-matrix interactions. Galectin-7 is specifically expressed in stratified epithelia and epidermis, but barely detected in epidermal tumors and absent from squamous carcinoma cell lines. Galectin-7 is a p53-induced protein and is abnormally expressed in variety of tumors such as melanoma, lung cancer and colon cancer. In UVB-induced sunburn keratinocytes, the expression of Galectin-7 is markedly increased due to the genotoxic stress-induced p53 activation. Both positive and negative regulatory roles of Galectin-7 have been reported in tumorigenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human Galectin-7
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820164 (BioLegend Cat. No. 866301)
AB_2820164 (BioLegend Cat. No. 866302)

Antigen Details

Structure
Human Galectin-7 is a 136 amino acid protein with a molecular mass of 15 kD.
Distribution

Tissue distribution: Stratified squamous epithelium
Cellular distribution: Nucleus, extracellular region or secreted, and cytoplasm

Function
Galectin-7 is involved in the cell-cell and cell-matrix interactions.
Ligand/Receptor
Carbohydrates
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Adhesion, Cell Biology, Neurodegeneration
Molecular Family
Adhesion Molecules, Soluble Receptors
Antigen References
  1. Bernerd F. et al. 1999. Proc Natl Acad Sci U S A. 96(20):11329-34.
  2. Madsen P. et al. 1995. J Biol Chem. 270(11):5823-9.
  3. Biron-Pain K. et al. 2013. PLoS One. 8(5):e63307. 
Gene ID
3963 View all products for this Gene ID
UniProt
View information about Galectin-7 on UniProt.org
Go To Top Version: 2    Revision Date: 12/02/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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