- Clone
- R24 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GD3S, SIAT8, Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, SIAT8-A, ganglioside GD3 synthase, sialytransferase St8Sia I, alpha-2,8-sialyltransferase 8A, disialoganglioside (GD3) synthase, ganglioside-specific alpha-2,8-polysialyltransferase
- Previously
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Signet Catalog# 8110-01
Covance Catalog# SIG-38110
- Isotype
- Mouse IgG3, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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917701 | 1 mL | $392 |
GD3, also known as MEL-1, is a ganglioside found in almost all melanomas and is an important biomarker for malignant melanoma. GD3 is found in melanocytes and other cells that originate in the neuroectoderm. Anti-GD3 antibodies have been shown to induce partial remission of tumor growth due to the proliferation of cytotoxic lymphocytes.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against SK-MEL-28 melanoma cell line.
- Formulation
-
Phosphate-buffered solution with BSA + 0.09% NaN3.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- The antibody was purified by affinity chromatography.
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC-F - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 with for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Tissue Sections: The preferred specimens for this antibody are frozen tissues and/or cytocentrifuge preparations fixed 10 minutes in 4°C acetone.
Pretreatment: Not required
Incubation: 20-60 minutes at room temperature - Application Notes
-
This antibody is effective in immunohistochemistry (IHC).
Positive tissue (human): Melanoma
MEL-1 (clone R24) recognizes disialoganglioside GD3, a prominent cell surface marker of human melanoma cells. -
Application References
(PubMed link indicates BioLegend citation) -
- Meidenbauer N, et al. 2004. Cancer Res. 64: 6319.
- Kasahara K, et al. 2000. J. Biol. Chem. 275: 34701.
- Watarai S, et al. 1991. J. Biochem. 110: 889.
- Yuyama K, et al. 2007. J. Biol. Chem. 282: 26392.
- Product Citations
-
- RRID
-
AB_2565200 (BioLegend Cat. No. 917701)
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All GD3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GD3 (MEL-1) | R24 | IHC-F |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-GD3 (MEL-1)
Staining of MEL-1 (R24) on frozen human melanoma tissue.
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