- Clone
- L21/32 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GluA2/GluR2 glutamate receptor, Glutamate receptor 2, GluR2, gluR-2, glutamate receptor B, AMPA-selective glutamate receptor 2
- Previously
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Covance Catalog# MMS-5144
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_L21slash32_Purified_GluR2_Antibody_1_WB_071818 1_L21slash32_Purified_GluR2_Antibody_1_WB_071818](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L21slash32_Purified_GluR2_Antibody_1_WB_071818.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-GluR2 antibody (clone L21/32). Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human brain membrane lysate; Lane 3, 7: 20 µg of mouse brain membrane lysate; Lane 4, 8: 20 µg of rat brain membrane lysate. The blots were incubated with 10 µg/mL of clone L21/32 (left) or mouse IgG1 (right) overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm. -
IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm. -
IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
810502 | 25 µL | $83 | ||||
810501 | 100 µL | $165 |
Glutamate receptor 2 (GluR2), also known as glutamate receptor B, is a subunit of AMPA receptors (AMPARs) involved in fast excitatory synaptic transmission. GluR2 regulates the permeability of an AMPAR to sodium, potassium, and calcium, and the absence of GluR2 allows AMPARs to be blocked by polyamines.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 834-883 of rat GluA2/GluR2 glutamate receptor.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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It does not cross-react with GluA1/GluR1, GluA3/GluR3, or GluA4/GluR4.
- Product Citations
-
- RRID
-
AB_2734564 (BioLegend Cat. No. 810502)
AB_2564751 (BioLegend Cat. No. 810501)
Antigen Details
- Structure
- Expected Molecular Weight: 90 kD
- Cell Type
- Glutamatergic Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 29627 View all products for this Gene ID
- UniProt
- View information about GluA2/GluR2 glutamate receptor on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All GluA2/GluR2 glutamate receptor Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GluR2 | L21/32 | WB,IHC-P |
Alexa Fluor® 594 anti-GluR2 | L21/32 | IHC-P |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-GluR2
Western blot of purified anti-GluR2 antibody (clone L21/32).... IHC staining of anti-GluR2 antibody (clone L21/32) on formal... IHC staining of anti-GluR2 antibody (clone L21/32) on formal... IHC staining of anti-GluR2 antibody (clone L21/32) on formal... -
Alexa Fluor® 594 anti-GluR2
IHC staining of Alexa Fluor® 594 anti-GluR2 antibody (clone ...
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