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Purified anti-GlyT2 (SLC6A5) Antibody

Pricing & Availability
Clone
A18174E (See other available formats)
Regulatory Status
RUO
Other Names
GLYT-2, NET1, HKPX3, Glycine Transporter 2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A18174E_PURE_GlyT2_Antibody_1_122619.png
Western blot of purified anti-GlyT2 (SLC6A5) antibody (clone A18174E). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18174E_PURE_GlyT2_Antibody_1_122619.png
    Western blot of purified anti-GlyT2 (SLC6A5) antibody (clone A18174E). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18174E_PURE_GlyT2_Antibody_2_122619.png
    ICC staining of purified anti-GlyT2 (SLC6A5) antibody (clone A18174E) on primary mouse neurons. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1, Cat. No. 801203). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
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870801 25 µg $106
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870802 100 µg $276
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Description

Glycine transporter 2 (GlyT2) protein is encoded by the SLC6A5 gene. It is one of the major glycine transporters that is expressed predominantly in the brain stem and spinal cord, and is frequently used as cellular marker for glycinergic neurons in the central nervous system. Mutations in the SLC6A5 gene have been clinically linked to hyperekplexia 3, which has been thought to be caused by disruption of inhibitory neuronal transmission.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to a.a. 182-199 of mouse SLC6A5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1 - 5 µg/mL. For immunocytochemistry, a concentration range of 2 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820198 (BioLegend Cat. No. 870801)
AB_2820198 (BioLegend Cat. No. 870802)

Antigen Details

Structure
GlyT2 is a 797 amino acid protein with predicted and observed molecular mass of ~87 kD.
Distribution

Tissue Distribution: Brain
Cellular Distribution: Plasma Membrane

Function
Glycine transport and recycling
Ligand/Receptor
Glycine
Cell Type
Mature Neurons, Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Neurotransmitter Receptor Trafficking/Endocytosis, Presynaptic proteins
Antigen References
  1. Zafra F, et al. 2017. Adv Neurobiol. 16:55.
  2. Lopez-Corcuera B, et al. 2017. Adv Neurobiol. 16:13.
  3. Vandenberg RJ, et al. 2014. Trends Pharmacol Sci. 35(8):423.
Gene ID
9152 View all products for this Gene ID 171148 View all products for this Gene ID
UniProt
View information about GlyT2 on UniProt.org
Go To Top Version: 1    Revision Date: 12/26/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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