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Purified anti-GSK-3α Antibody

Pricing & Availability
Clone
W15183A (See other available formats)
Regulatory Status
RUO
Other Names
Glycogen synthase kinase alpha, GSK-3 alpha
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W15183A_PURE_GSK-3alpha_Antibody_1_032019
Total cell lysates (15 µg total protein) from HEK293T (WT) and HEK293T GSK3α KD cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-GSK3α Antibody, clone W15183A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W15183A_PURE_GSK-3alpha_Antibody_1_032019
    Total cell lysates (15 µg total protein) from HEK293T (WT) and HEK293T GSK3α KD cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-GSK3α Antibody, clone W15183A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W15183A_PURE_GSK-3alpha_Antibody_2_032019
    Total cell lysates (15 µg total protein) from HeLa (reduced expression control), HEL, U87-MG and A549 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-GSK3α Antibody, clone W15183A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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688902 100 µg $288
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Description

Glycogen Synthase Kinase 3 Alpha (GSK-3α) is a serine/threonine protein kinase which mediates the control of several regulatory proteins involved in glucose homeostasis, including glycogen synthase, and transcription factors such as JUN. It also plays a role in the WNT and PI3K signaling pathways. GSK-3α contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting glycogen synthase (GYS1 or GYS2) activity and mediates the development of insulin resistance. In Wnt signaling, GSK-3α regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. GSK-3α is also reported to facilitate the generation of amyloid precursor protein (APP)-derived amyloid plaques found in Alzheimer disease. To date, there are six GSK-3α mutant mouse lines generated, these stains express various abnormal phenotypes in growth, size, mortality, aging, adipose morphology, homeostasis and metabolism, muscle and the nervous system.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human GSK-3α recombinant protein (251-483 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629652 (BioLegend Cat. No. 688902)

Antigen Details

Structure
483 amino acids with a predicted molecular weight of 51 kD. GSK-3α is a serine/threoenine kinase.
Distribution

Cytoplasm.

Function
GSK-3α is a serine/threonine kinase, mediating signals in the WNT and the PI3K pathways. GSK-3α is implicated in the regulation of glucose metabolism and homeostasis.
Interaction
Interacts with ARRB2, AXIN1, CTNNB1, and CTNND2.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Park SA, et al. 2016. PLoS One 11:e0153075.
2. Ye C, et al. 2015. J. Neurochem. 133:273.
3. Grassilli E, et al. 2014. PLoS One 9:e100947.
4. Nekova TS, et al. 2014. Br. J. Haematol. 167:272.
5. Zhou J, et al. 2013. J. Clin. Invest. 123:1821.
6. Jaworski T, et al. 2011. Nature 480:e4.

Gene ID
2931 View all products for this Gene ID
UniProt
View information about GSK-3alpha on UniProt.org
Go To Top Version: 3    Revision Date: 03/20/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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