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Purified anti-GSK-3α/β Antibody

Pricing & Availability
Clone
W17173C (See other available formats)
Regulatory Status
RUO
Other Names
Glycogen Synthase Kinase, Glycogen Synthase Kinase 3 Alpha, Glycogen Synthase Kinase Beta, GSK3A, GSK3B
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17173C_PURE_GSK-3alpha-beta_Antibody_1_070319.png
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a .45 µm PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-GSK-3α/β Antibody, clone W17173C, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • W17173C_PURE_GSK-3alpha-beta_Antibody_1_070319.png
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a .45 µm PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-GSK-3α/β Antibody, clone W17173C, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • W17173C_PURE_GSK-3alpha-beta_Antibody_2_070319.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of clone W17173C or with Purified Rat IgG2a, κ Isotype Control Antibody (Cat. No. 400502). The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a .45 µm PVDF membrane, and probed with a separate anti-GSK-3 α/β antibody raised with a different immunizing GSK sequence. Lane M: Molecular Weight marker.
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615001 25 µg $101
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615002 100 µg $253
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Description

GSK-3α/β is a ubiquitously expressed serine/threonine kinase that can phosphorylate a wide range of substrates. While GSK-3 was initially shown to regulate energy metabolism through phosphorylation of glycogen synthase, it has been shown that the enzyme has a wide variety of substrates in vivo and regulates signaling pathways that control cell growth, development,  apoptosis, and oxidative stress responses. Each isoform if encoded by separate alleles; the isoforms show 66% homology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant full-length GSK-3β
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes both a and ß isoforms of GSK-3 by both WB and IP.

This clone failed to stain PFA-fixed HeLa cells that were permeabilized with methanol or Triton-X. We therefore do not recommend using this clone for ICC.

RRID
AB_2814452 (BioLegend Cat. No. 615001)
AB_2814452 (BioLegend Cat. No. 615002)

Antigen Details

Structure
GSK-3α is a 483 amino acid protein with a predicted molecular weight of 51 kD; GSK-3β is a 420 amino acid protein with a predicted molecular weight of 47 kD.
Distribution

Both isoforms ubiquitously expressed
GSK-3α: cytosol; GSK-3β: nucleus

Function
Cell Signaling
Antigen References
  1. Imazu M, et al. 1984. J. Biol. Chem. 259: 1813.
  2. Cross DA, et al. 1995. Nature. 378: 785.
  3. Kazi A, et al. 2018. Nat. Commun. 9: 5154.
Gene ID
2931 View all products for this Gene ID 2932 View all products for this Gene ID
UniProt
View information about GSK-3alpha/beta on UniProt.org
Go To Top Version: 1    Revision Date: 07/03/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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