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Purified anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# PRB-101P)

Pricing & Availability
Clone
Poly9023 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# PRB-101P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Purified_HA.11_WB_021315
Western Blot showing specificity of HA.11, clone Poly9023, at 1:1000 and 1:2000. Lane 1: Molecular weight marker. Lane 2: CHO Clone 6. Lane 3: BSA negative control.
  • Purified_HA.11_WB_021315
    Western Blot showing specificity of HA.11, clone Poly9023, at 1:1000 and 1:2000. Lane 1: Molecular weight marker. Lane 2: CHO Clone 6. Lane 3: BSA negative control.
Cat # Size Price Quantity Check Availability Save
902303 25 µL $89
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902301 200 µL $448
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902302 500 µL $706
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical data sheet

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal antibody HA.11 was raised against the twelve amino acid sequence CYPYDVPDYASL.
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IP
ChIP, ICC, Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000*
• IP: 1:150

Application Notes

This antibody is effective in immunoblotting (WB) and immunoprecipitation (IP) of tagged proteins.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
  2. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  3. Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
  4. Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
  5. Yeo C, Whitman M. 2001. Mol Cell 7:949.
  6. Sander TL, et al. 2000. J Biol Chem.:12857. (WB)
  7. Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
  8. Hewawasam G, et al. 2010. Mol Cell. 40:444. (ChIP, WB) PubMed
  9. Hurov JB, et al. 2004. Curr Biol. 14:736. (IF)
  10. Shieh AW, et al. 2013. PLoS One. 8:75337. (WB, IF) PubMed
  11. Hackenbeck T, et al. 2009. Cell Cycle. 8:1386. (ICC)
  12. Satheshkumar PS, et al. 2013. J Virol. 87:10710.
  13. Luo R, et al. 2009. Cell Signal. 21:1169. (WB) PubMed
  14. Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed
Product Citations
  1. Yuan G, et al. 2022. ACS Synth Biol. 11:2513. PubMed
  2. Suzuki R, et al. 2023. Microbiol Immunol. 67:22. PubMed
  3. Pal D, et al. 2023. iScience. 26:106274. PubMed
  4. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  5. Xiao X, et al. 2023. Nat Commun. 14:2859. PubMed
  6. Yuan G, et al. 2023. Commun Biol. 6:567. PubMed
  7. Chalker A 2013. PLoS One. 8:e75337. PubMed
  8. Rinschen M, et al. 2016. Hum Mol Genet. 25: 1328-1344. PubMed
  9. Luo R, et al. 2009. Cell Signal. 21:1169-1179. PubMed
  10. Berg T, et al. 2010. J Biol Chem. 285:35255-32566. PubMed
  11. Pal D, et al. 2022. Commun Biol. 5:646. PubMed
  12. Morales L, et al. 2022. Front Neural Circuits. 16:831074. PubMed
  13. Ho TYH, et al. 2021. Nat Commun. 12:2200. PubMed
  14. Wang Q, et al. 2019. Biomolecules. 9:4. PubMed
  15. Tsao N, et al. 2016. Biochem J. 473: 1237 - 1245. PubMed
  16. Liu X, et al. 2021. Nat Commun. 12:4427. PubMed
  17. Martin CK, et al. 2022. Viruses. 14:. PubMed
  18. Szilágyi SS, et al. 2022. BMC Biol. 20:50. PubMed
  19. Nissim S, et al. 2019. Nat Genet. 51:1308. PubMed
  20. Dafinger C, et al. 2018. Exp Mol Med. 50:75. PubMed
  21. Judith D, et al. 2019. J Cell Biol. 218:1634. PubMed
  22. Bartram M, et al. 2016. Hum Mol Genet. 25: 1152 - 1164. PubMed
  23. Hewawasam G, et al. 2010. Mol Cell. 40:444-454. PubMed
  24. Choi Y, et al. 2019. Nat Microbiol. 4:429. PubMed
  25. Choi YJ, et al. 2019. Nat Immunol. 20:276. PubMed
  26. Coulombe P et al. 2019. Cell reports. 27(6):1769-1780 . PubMed
  27. Patchett S, et al. 2021. Cell Rep. 36:109754. PubMed
  28. Robles‐Valero J et al. 2017. Cancer cell. 32(5):608-623 . PubMed
RRID
AB_2565018 (BioLegend Cat. No. 902303)
AB_2565018 (BioLegend Cat. No. 902301)
AB_2565018 (BioLegend Cat. No. 902302)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Other Formats

View All HA.11 Reagents Request Custom Conjugation
Description Clone Applications
Anti-HA Tag Poly9023 WB,IP
Purified anti-HA.11 Epitope Tag Poly9023 WB,IP,ChIP,ICC,Purification
Go To Top Version: 6    Revision Date: 01/25/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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