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Purified anti-Hepatitis B Surface Antigen (HBsAg) Antibody

Pricing & Availability
Clone
A10F1 (See other available formats)
Regulatory Status
RUO
Other Names
HBsAg, Major surface protein, Large S protein, Hepatitis B Surface Antigen, ayw subtype, Australian antigen
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A10F1_PURE_Hepatitis_B_Antibody_IHC_102615
IHC staining of purified anti-Hepatitis B Surface Antigen (HBsAG) antibody (clone A10F1) on formalin-fixed paraffin-embedded human Hepatitis B infected liver tissue. The tissue was incubated with 2 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • A10F1_PURE_Hepatitis_B_Antibody_IHC_102615
    IHC staining of purified anti-Hepatitis B Surface Antigen (HBsAG) antibody (clone A10F1) on formalin-fixed paraffin-embedded human Hepatitis B infected liver tissue. The tissue was incubated with 2 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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932302 100 µg $288
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Description

Hepatitis B is a liver infection caused by the Hepatitis B virus (HBV). Hepatitis B surface antigen (HBsAg) contains the large (L), middle (M), and small (S) surface proteins of HBV. HBsAg can be detected at high levels in serum during acute or chronic HBV infection. The body normally produces antibodies to HBsAg as part of the normal immune response to infection. HBsAg is the antigen used to make the HBV vaccine and is also a diagnostic marker.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Hepatatis B virus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified HBsAg from human HBsAg-positive serum.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Huang T, et al. 2005. J. Biol. Chem. 280:27742. (WB)
  2. Wang H, et al. 2003. Am. J. Pathol. 163:2441. (WB)
  3. Shih H, et al. 2008. J. Virol. 82:2250. (WB)
  4. Shih H, et al. 2012. J. Virol. 86:9044. (WB)
  5. Hsu S, et al. 2004. J. Virol. 78:6. (WB)
Product Citations
  1. Giadans CG, et al. 2019. J Viral Hepat. 26:727. PubMed
  2. Biswas S, et al. 2022. Nat Commun. 13:2995. PubMed
RRID
AB_2566428 (BioLegend Cat. No. 932302)

Antigen Details

Structure
Hepatitis B surface antigen (HBsAg) contains the large (L), middle (M), and small (S) surface proteins of Hepatitis B virus (HBV).
Distribution

HBsAg is on the surface of HBV that is present in bodily fluids and hepatocytes of infected individuals.

Function
HBsAg plays a key role in HBV's infection of hepatocytic cells.
Interaction
Hepatocytes.
Ligand/Receptor
Several hepatocytic receptors including asialoglycoprotein receptors and Annexin V.
Biology Area
Cell Biology
Antigen References

1. Toita B, et al. 2015. World J. Gastroenterol. 24:7400-7411.

Gene ID
944569 View all products for this Gene ID
UniProt
View information about HBsAg on UniProt.org
Go To Top Version: 3    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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