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Purified anti-HIF1A Antibody

Pricing & Availability
Clone
W22058A (See other available formats)
Regulatory Status
RUO
Other Names
hypoxia inducible factor 1 alpha (Hif1α), ARNT-interacting protein, basic-helix-loop-helix-PAS protein MOP1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W22058A_PURE_HIFIA_IHC-P_022924
IHC staining of Purified anti-HIF1A (clone W22058A) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-HIF1A (clone W22058A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X objective. Scale bar: 50 µm
  • A. W22058A_PURE_HIFIA_IHC-P_022924
    IHC staining of Purified anti-HIF1A (clone W22058A) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-HIF1A (clone W22058A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X objective. Scale bar: 50 µm
  • B. W22058A_PURE_HIFIA_WB_022924
    Whole cell extracts (15 µg total protein) from HeLa cells untreated or treated with 200 µM CoCl2 overnight were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-HIF1A (clone W22058A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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633351 25 µg $170
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633352 100 µg $455
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Description

HIF1, hypoxia induced factor 1, is a heterodimeric basic helix-loop-helix transcription factor composed of HIF1α and HIF1β. Under normoxic conditions, HIF1α undergoes prolyl hydroxylation which leads to degradation of HIF1α by the proteasome. Oxygen-starvation inhibits prolyl hydroxylation and prevents proteasomal degradation of HIF1α, leading to stabilization of HIF1. HIF1α contains a nuclear localization sequence, and following nuclear translocation HIF1α binds to the core DNA sequence 5'-[AG]CGTG-3' within the hypoxia responsive element (HRE). HIF1 is a master regulator of the response to hypoxia and induces transcription of multiple genes including VEGF, glucose transporters, and proteins that aid in adaptation to hypoxic conditions. HIF1 protein is also involved in the regulation of angiogenesis, erythropoiesis, and energy metabolism.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Fragment of human HIF1A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.125 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone W22058A) is not recommended for immunocytochemistry (ICC). 

For IHC-P, antigen retrieval using Tris-EDTA pH 9.0 buffer (10mM Tris, 1mM EDTA) is recommended. Antigen retrieval with Sodium Citrate H.I.E.R. (Cat. No. 928502) is not recommended due to low signal.

RRID
AB_3097615 (BioLegend Cat. No. 633351)
AB_3097615 (BioLegend Cat. No. 633352)

Antigen Details

Structure
HIF1A is an 826 amino acid protein with a predicted molecular weight of 92.7 kD.
Distribution

Cytoplasmic in normoxia, nuclear in response to hypoxia

Function
Transcription factor, oxygen sensor
Interaction
Forms a dimer with Hif1β. Prolylation conducted by prolyl hydroxylase (PHD) and HIF prolyl hydroxylase (HPH), and subsequent degradation is mediated by von Hippel-Lindau tumor suppressor (VHL).
Antigen References
  1. Wang GL, et al. 1995. Proc Natl Acad Sci USA. 92:5510-4. 
  2. Lee C, et al. 2020. Biomed J. 43:434-437. 
  3. Rashid M, et al. 2021. Gene. 798:145796.
Gene ID
3091 View all products for this Gene ID
UniProt
View information about HIF1A on UniProt.org
Go To Top Version: 1    Revision Date: 02/29/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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