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Purified anti-Histone H2B Antibody

Pricing & Availability
Clone
W17025A (See other available formats)
Regulatory Status
RUO
Other Names
Histone Cluster 1, H2bb, H2B Histone Family-Member F, H2BFF, Histone H2B.1, H2B/F
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
a-W17025A_PURE_Histone_H2B_Antibody_WB_011718
Total cell lysates (15 µg protein) from HeLa (lane 1), HaCaT (lane 2), and NIH/3T3 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with the indicated concentration (µg/mL) of purified anti-Histone H2B (Clone W17025A) or a competitor’s clone used at the manufacturer’s recommended concentration (far right). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) for clone W17025A and HRP goat anti-mouse IgG (Cat. No. 405306) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular weight ladder.
  • a-W17025A_PURE_Histone_H2B_Antibody_WB_011718
    Total cell lysates (15 µg protein) from HeLa (lane 1), HaCaT (lane 2), and NIH/3T3 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with the indicated concentration (µg/mL) of purified anti-Histone H2B (Clone W17025A) or a competitor’s clone used at the manufacturer’s recommended concentration (far right). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) for clone W17025A and HRP goat anti-mouse IgG (Cat. No. 405306) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular weight ladder.
  • b-W17025A_PURE_Histone_H2B_Antibody_WB_2_081619
    Western blot of purified anti-Histone H2B antibody (clone W17025A). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.25 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
  • c-W17025A_PURE_Histone_H2B_Antibody_3_100319
    IHC staining of purified anti-Histone H2B antibody (clone W17025A) on formalin-fixed paraffin-embedded Drosophila brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti- rat IgG (Cat No. 405422) for one hour at room temperature. Nuclei were counterstained with DAPI. A shows single channel image of purified anti-histone H2B antibody and B shows merged images with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
606301 25 µg $101
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606302 100 µg $253
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Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histones play central roles in transcription regulation, DNA repair, DNA replication and chromosomal stability. There are many variants of histones H2A and H2B. Specific H2A and H2B variants may preferentially associate with each other resulting in different combinations of variants and leading to the increased combinatorial complexity of nucleosomes. When being catalyzed by a series of histone modifying enzymes, histones may undergo various post-translational modifications such as acetylation, methylation, phosphorylation, ubiquitylation and SUMOylation. The dysregulation of histone modifying enzymes will alter the histone post-modification patterns and cause diverse diseases including cancers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Drosophila
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human H2B1A peptide (111-127 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml (1:250-2000). For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Reactivity to Drosophila was only verified with the purified format.

Product Citations
  1. Hayashi M, et al. 2021. Viruses. 13:. PubMed
RRID
AB_2728464 (BioLegend Cat. No. 606301)
AB_2728464 (BioLegend Cat. No. 606302)

Antigen Details

Structure
126 amino acids with a predicted molecular weight of approximately 14kD but observed at approximately 17kD as the highly positively charged Histone protein tends to migrate slower on SDS-PAGE gel.
Distribution

Eukaryotic cells and nucleus.

Function
Histone H2B is one of the building blocks of nucleosome. It plays a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction
Histone H2A
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication
Antigen References

1. Ma F, Zhang CY. 2016. Expert Rev. Mol. Diagn. 16:297.
2. Li J, et al. 2016. Biomed. Rep. 4:293.
3. Shaytan AK, et al. 2015. Curr. Opin. Struct. Biol. 32:48.
4. Wright DE, Kao CF. 2015. Epigenetics 10:122.
5. Kim J, et al. 2009. Cell. 137:459.
6. Cheung WL, et al. 2003. Cell. 113:507.

Gene ID
3018 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org
Go To Top Version: 4    Revision Date: 10/03/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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