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Purified anti-Hsp70 Antibody

Pricing & Availability
Clone
W27 (See other available formats)
Regulatory Status
RUO
Other Names
Heat shock protein 70
Isotype
Mouse IgG2a, λ
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Product Citations
publications
A_W27_PURE_HSP70_Antibody_1_WB_082616
Total cell lysates (15 µg protein) from Jurkat, NTERA-2, NIH3T3 and HEK293 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Hsp70 antibody (clone W27). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-β-actin Antibody was used as a loading control.
  • A_W27_PURE_HSP70_Antibody_1_WB_082616
    Total cell lysates (15 µg protein) from Jurkat, NTERA-2, NIH3T3 and HEK293 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Hsp70 antibody (clone W27). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-β-actin Antibody was used as a loading control.
  • B_W27_PURE_Hsp70_Antibody_2_PE_082616
    Human cervical cancer cell line, HeLa, intracellularly stained with purified W27 conjugated with PE.
  • C_W27_PURE_Hsp70_IF_032318
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-Hsp70 Antibody (clone W27) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG (Cat # 405326) in blocking buffer for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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648001 25 µg $124
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648002 100 µg $293
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Description

Hsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits immunomodulatory effects on innate and acquired immunity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Hsp70 protein from HeLa cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB, ICFC - Quality tested
ICC - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Fong J, et al. 2015. EMBO J. 34: 2775 - 2788. PubMed
  2. Zhang K, et al. 2021. Neuron. 109(12):1949-1962.e6. PubMed
  3. Våtsveen TK, et al. 2018. J Hematol Oncol. 11:23. PubMed
  4. Harris IS et al. 2019. Cell metabolism. 29(5):1166-1181 . PubMed
  5. Hashimoto M, et al. 2022. Front Neurosci. 16:948517. PubMed
RRID
AB_2028531 (BioLegend Cat. No. 648001)
AB_2028531 (BioLegend Cat. No. 648002)

Antigen Details

Structure
Hsp proteins are the most conserved proteins present in both prokaryotes and eukaryotes. Human HSp70 contains multiple isoforms. Hsp70s consisit of three structural domains, a 44Kd amino-terminal ATPase domain (NBD), followed by an 18 Kd substrate
Distribution

Ubiquitous, cytoplasmic and nuclear

Function
Molecular chaperrones assisting in the folding of proteins and preventing protein aggregation
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
Antigen References

1. Sharma D, et al. 2009. Protein Peptide Lett. 16:571.
2. Johnson JD, et al. 2006. J. Leuk. Biol. 79:425.
3. Bukau B, et al. 1998. Cell 92:351.

Gene ID
3308 View all products for this Gene ID
UniProt
View information about Hsp70 on UniProt.org
Go To Top Version: 6    Revision Date: 08/31/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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