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Purified anti-human AXL Antibody

Pricing & Availability
Clone
S21005C (See other available formats)
Regulatory Status
RUO
Other Names
ARK, AXL receptor tyrosine kinase, AXL transforming sequence/gene, AXL oncogene, JTK11, Tyro7, tyrosine-protein kinase receptor UFO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S21005C_PURE_AXL_Antibody_FC_042823
Human peripheral blood mononuclear cells were stained with purified anti-human AXL (clone S21005C) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG PE. Cells were then co-stained for lineage of human CD3/CD19/CD14/CD15 FITC and CD303 APC. Data shown was gated lineage negative population.
  • S21005C_PURE_AXL_Antibody_FC_042823
    Human peripheral blood mononuclear cells were stained with purified anti-human AXL (clone S21005C) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG PE. Cells were then co-stained for lineage of human CD3/CD19/CD14/CD15 FITC and CD303 APC. Data shown was gated lineage negative population.
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386502 100 µg $235
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Description

AXL, also known as ARK or UFO, is a widely expressed glycoprotein in the TAM (Tyro3/Dtk, AXL, and Mer) receptor tyrosine kinase family. AXL is highly expressed in solid cancers. It is also expressed on a small population of human dendritic cells that share characteristics of classical and plasmacytoid dendritic cells. AXL binds the vitamin K-dependent growth arrest specific 6 (Gas6) protein and induces receptor autophosphorylation and downstream signaling pathways that are related to cell proliferation, migration, or apoptosis. AXL is also involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Recent studies have also shown that AXL and Mer are both linked to Alzheimer's disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S21005C recognizes a different epitope compared to clone S21005B.

Clone S21005C does not block GAS6 induced AKT and ERK phosphorylation in H1299 cells.

RRID
AB_2941569 (BioLegend Cat. No. 386502)

Antigen Details

Structure
Transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain
Distribution

Highly expressed in solid cancers and also expressed on human blood plasmacytoid dendritic cells and myeloid dendritic cells

Function
Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that are related to cell proliferation, migration, or apoptosis. It is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Recent studies have shown that AXL and Mer are both linked to Alzheimer's disease.
Ligand/Receptor
Gas6 and Protein S
Cell Type
Dendritic cells, NK cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cardiovascular Biology, Cell Death, Cell Proliferation and Viability, Immuno-Oncology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, Cytokines/Chemokines
Antigen References
  1. Holland S, et al. 2005. Canc Res. 65:9294-303.
  2. Lee WP, et al. 2002. Oncogene. 21:329-36.
  3. Demarchi F, et al. 2001. J Biol Chem. 276:31738-44.
Gene ID
558 View all products for this Gene ID
UniProt
View information about AXL on UniProt.org
Go To Top Version: 1    Revision Date: 04/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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