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Purified anti-human CCX-CKR (CCRL1) Antibody

Pricing & Availability
Clone
13E11 (See other available formats)
Regulatory Status
RUO
Other Names
ACKR4, CC-CKR-11, VSHK1, PPR1, CCBP2, CCR11, CKR-11, CCXCKR
Isotype
Mouse IgG2b, κ
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Product Citations
publications
13E11_Purified_CCRL1_Antibody_FC_022014.jpg
Human CCX-CKR (CCRL1)-transfected bovine coronary artery endothelial cell line (B300) was stained with purified anti-human CCRL1 (clone 13E11, filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • 13E11_Purified_CCRL1_Antibody_FC_022014.jpg
    Human CCX-CKR (CCRL1)-transfected bovine coronary artery endothelial cell line (B300) was stained with purified anti-human CCRL1 (clone 13E11, filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
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362102 100 µg $241
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Description

Human CCX-CKR, also known as CCRL1, is a 40 kD, G-protein-coupled, seven transmembrane receptor. CCRL1 is a decoy receptor for CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25 that regulates chemotaxis of lymphocytes and cancer cells. Chemokine binding by CCX-CKR causes its internalization, reducing levels of available chemokine, and thus regulating migration of lymphocytes and cancerous cells. CCX-CKR is expressed by stromal cells of skin-draining lymph nodes, thymic epithelial cells, and immature dendritic cells (DCs). A low CCX-CKR expression in tumor cells correlates with a poor survival rate of breast cancer patients.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length CCX-CKR cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IP, Agonist - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation of CCX-CKR from cell lysates1, induction of CCX-CKR internalization1 and immunofluorescence1. 13E11 recognizes an epitope located within the first 14 amino acids of CCX-CKR.

Application References

(PubMed link indicates BioLegend citation)
  1. Takatsuka, S. et al. 2011. J. Pharmacol. Toxicol. Methods. 63:250. (IF, IP, Agonist)
Product Citations
  1. Bryce S, et al. 2016. J Immunol. 196: 3341-3353. PubMed
  2. Matti C, et al. 2020. Front Immunol. 11:720. PubMed
RRID
AB_2563201 (BioLegend Cat. No. 362102)

Antigen Details

Structure
G-protein-coupled seven transmembrane receptor, 40 kD.
Distribution

Stromal cells of skin-draining lymph nodes, thymic epithelial cells, immature DCs.

Function
C-C type chemokine scavenger and decoy receptor, reduces chemokine availability by sequestering them inside the cell.
Interaction
β-arrestin.
Ligand/Receptor
CCL2, CCL8, CCL13, CCL19, CCL21, and CCL25.
Cell Type
Dendritic cells, Epithelial cells
Biology Area
Immunology
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Watts AO, et al. 2013. J. Biol. Chem. 288:7169. 
2. Comerford I, et al. 2010. Blood. 116:4130.
 

Gene ID
51554 View all products for this Gene ID
UniProt
View information about CCX-CKR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/23/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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