Purified anti-human CD10 Maxpar Ready Antibody anti-CD10

Pricing & Availability

Clone: HI10a (See other available formats)
Regulatory Status: RUO
Workshop: V CD10.7
Other Names: Common acute lymphoblastic leukemia antigen (CALLA), Enkephalinase, Neutral endopeptidase, Neprilysin
Isotype: Mouse IgG1, κ
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Product Citations: publications

Hi10a_Purified_CD10_CyTOF_112213 — Human PBMCs (top) and human NALM-6 pre-B cells (bottom) stained with 156Gdanti-CD10 (HI10a). Data provided by DVS Sciences.

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312223

100 µg

$141

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Description

CD10 is a 100 kD neutral endopeptidase and a member of the metalloprotease family. It is a type II transmembrane protein also known as common acute lymphoblastic leukemia antigen (CALLA), enkephalinase, and neprilysin. CD10 is expressed on B cell precursors, T cell precursors, and neutrophils. CD10 is involved in B cell development and has been shown to bind opioid enkephalins, bradykinin, angiotensins I and II, and other biologically active peptides.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Capuchin monkey, Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2562828 (BioLegend Cat. No. 312223)

Antigen Details

Structure: Type II transmembrane metalloprotease family, 100 kD
Distribution: B cell precursors, T cell precursors, neutrophils
Function: Zinc-binding metalloproteinase, B cell development
Ligand/Receptor: Biologically active peptides including opioid enkephalins, bradykinin, angiotensions I & II
Cell Type: B cells, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Shipp M, et al. 1993. Blood 82:1052.
2. Lu B, et al. 1995. J. Exp. Med. 181:2271.
Gene ID: 4311 View all products for this Gene ID
UniProt: View information about CD10 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD10 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD10	HI10a	FC,CyTOF®,IHC-F
PE anti-human CD10	HI10a	FC
PE/Cyanine5 anti-human CD10	HI10a	FC
FITC anti-human CD10	HI10a	FC
APC anti-human CD10	HI10a	FC
APC/Cyanine7 anti-human CD10	HI10a	FC
PE/Cyanine7 anti-human CD10	HI10a	FC
PerCP/Cyanine5.5 anti-human CD10	HI10a	FC
Brilliant Violet 421™ anti-human CD10	HI10a	FC
Brilliant Violet 510™ anti-human CD10	HI10a	FC
Brilliant Violet 605™ anti-human CD10	HI10a	FC
Purified anti-human CD10 (Maxpar® Ready)	HI10a	FC,CyTOF®
Brilliant Violet 711™ anti-human CD10	HI10a	FC
PE/Dazzle™ 594 anti-human CD10	HI10a	FC
APC/Fire™ 750 anti-human CD10	HI10a	FC
APC anti-human CD10	HI10a	FC
PE anti-human CD10	HI10a	FC
FITC anti-human CD10	HI10a	FC
TotalSeq™-A0062 anti-human CD10	HI10a	PG
TotalSeq™-C0062 anti-human CD10	HI10a	PG
APC/Fire™ 750 anti-human CD10	HI10a	FC
TotalSeq™-B0062 anti-human CD10	HI10a	PG
PE/Cyanine7 anti-human CD10	HI10a	FC
PerCP/Cyanine5.5 anti-human CD10	HI10a	FC
Brilliant Violet 785™ anti-human CD10	HI10a	FC
TotalSeq™-D0062 anti-human CD10	HI10a	PG
GMP APC anti-human CD10	HI10a	FC
GMP FITC anti-human CD10	HI10a	FC
GMP PE/Cyanine7 anti-human CD10	HI10a	FC
GMP PE anti-human CD10	HI10a	FC
PE/Dazzle™ 594 anti-human CD10	HI10a	FC
GMP PerCP/Cyanine5.5 anti-human CD10	HI10a	FC
Brilliant Violet 650™ anti-human CD10	HI10a	FC
GMP PE/Dazzle™ 594 anti-human CD10	HI10a	FC
Brilliant Violet 750™ anti-human CD10	HI10a	FC
Spark Violet™ 423 anti-human CD10	HI10a	FC
Spark YG™ 593 anti-human CD10	HI10a	FC
Spark Blue™ 574 anti-human CD10	HI10a	FC
PE/Fire™ 640 anti-human CD10	HI10a	FC

Certificate of Analysis

Go To Top Version: 4 Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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