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Purified anti-human CD111 (Nectin-1) Antibody

Pricing & Availability
Clone
R1.302 (See other available formats)
Regulatory Status
RUO
Other Names
Poliovirus Receptor Related 1 Protein (PRR1), Nectin-1, HveC
Isotype
Mouse IgG1, κ
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Product Citations
publications
R1-302_PURE_CD111_Antibody_IHC-P_030519
Human paraffin embedded kidney tissue was stained with purified anti-human CD111 antibody and then goat anti-mouse Alexa Fluor® 488 (green) and DAPI (blue).
  • R1-302_PURE_CD111_Antibody_IHC-P_030519
    Human paraffin embedded kidney tissue was stained with purified anti-human CD111 antibody and then goat anti-mouse Alexa Fluor® 488 (green) and DAPI (blue).
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340402 100 µg $323
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Description

CD111, known as poliovirus receptor related 1 protein (PRR1), Nectin-1 and HveC, is a 75 kD type I transmembrane glycoprotein. A member of the nectin family and Ig gene superfamily, CD111 is broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells. CD111 functions as one of the herpes simplex virus 1 (HSV-1) and HSV-2 receptors and is an adhesion molecule involved in the formation of cell junctions.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Nectin-1 transfected NIH/3T3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
WB, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation, Western blot, immunohistochemical staining of frozen tissue sections, block HSV-1 and HSV-2 entry into cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Cocchi F, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:15700.
  2. Martinez WM and Spear PG. 2002. J. Virol. 76:7255.
  3. Reymond N,et al. 2004. J. Exp Med. 199:1331.
RRID
AB_2284683 (BioLegend Cat. No. 340402)

Antigen Details

Structure
75 kD type I transmembrane glycoprotein, Nectin family, Ig superfamily
Distribution

Myelomonocytic cells, megakaryocytes, CD34-positive stem cells, endothelial cells, epithelial cells, and neuronal cells.

Function
Adhesion, receptor for Herpes simplex Virus 1 (HSV-1) and HSV-2
Ligand/Receptor
Nectin-1, -2, -3, and -4, CD155, HSV-1 and HSV-2
Cell Type
Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Megakaryocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola h, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication.
2. Simpson SA, et al. 2005. J. Neuroviral. 11:208.
3. Akhtar J, et al. 2008. Ophthalmol. Vis. Sci. 49:4026.

Gene ID
5818 View all products for this Gene ID
UniProt
View information about CD111 on UniProt.org
Go To Top Version: 2    Revision Date: 11/23/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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