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Purified anti-human CD15 Recombinant Antibody

Pricing & Availability
Clone
QA19A61 (See other available formats)
Regulatory Status
RUO
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgG1, κ
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Product Citations
publications
a-QA19A61_PURE_CD15_Antibody_2_01052022
Human lysed washed blood stained with purified anti-human CD15 recombinant (clone QA19A61) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • a-QA19A61_PURE_CD15_Antibody_2_01052022
    Human lysed washed blood stained with purified anti-human CD15 recombinant (clone QA19A61) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • b-QA19A61_PURE_CD15_Antibody_1_01052022
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD15 recombinant (clone QA19A61) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Goat anti-mouse (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
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376302 100 µg $101
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910428 (BioLegend Cat. No. 376302)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Monocytes, Neural Stem Cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem J. 268:275-80.

 

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org
Go To Top Version: 1    Revision Date: 01/05/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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