Purified anti-human CD15 SSEA-1 Maxpar Ready Antibody anti-CD15

Pricing & Availability

Clone: W6D3 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Lewis X, 3-FAL, 3-FL, LNFP III, Lex, SSEA-1, X-hapten
Isotype: Mouse IgG1, κ
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Product Citations: publications

W6D3_Purified_CD15_CyTOF_112213 — Human PBMCs (blue) and RBC-lysed human whole blood cells (red) stained with 164Dy-anti-CD15 (W6D3). Data provided by DVS Sciences.

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323035

100 µg

$129

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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: WERI-RB-1 retinoblastoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2562831 (BioLegend Cat. No. 323035)

Antigen Details

Structure: Poly-N-acetyllactosamine
Distribution: Neutrophils, eosinophils, monocytes
Function: Adhesion
Cell Type: Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Stocks SC, et al. 1990. Biochem. J. 268:275.
Gene ID: 2526 View all products for this Gene ID
UniProt: View information about CD15 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD15 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 785™ anti-human CD15 (SSEA-1)	W6D3	FC
Purified anti-human CD15 (SSEA-1)	W6D3	FC,CyTOF®
FITC anti-human CD15 (SSEA-1)	W6D3	FC
PE anti-human CD15 (SSEA-1)	W6D3	FC
APC anti-human CD15 (SSEA-1)	W6D3	FC
Alexa Fluor® 488 anti-human CD15 (SSEA-1)	W6D3	FC
Alexa Fluor® 647 anti-human CD15 (SSEA-1)	W6D3	FC
PE/Cyanine5 anti-human CD15 (SSEA-1)	W6D3	FC
PerCP anti-human CD15 (SSEA-1)	W6D3	FC
PerCP/Cyanine5.5 anti-human CD15 (SSEA-1)	W6D3	FC
Pacific Blue™ anti-human CD15 (SSEA-1)	W6D3	FC
Alexa Fluor® 700 anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 510™ anti-human CD15 (SSEA-1)	W6D3	FC
PE/Cyanine7 anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 605™ anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 650™ anti-human CD15 (SSEA-1)	W6D3	FC
Purified anti-human CD15 (SSEA-1) (Maxpar® Ready)	W6D3	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 421™ anti-human CD15 (SSEA-1)	W6D3	FC
APC/Fire™ 750 anti-human CD15 (SSEA-1)	W6D3	FC
FITC anti-human CD15 (SSEA-1)	W6D3	FC
Pacific Blue™ anti-human CD15 (SSEA-1)	W6D3	FC
TotalSeq™-A0392 anti-human CD15 (SSEA-1)	W6D3	PG
Brilliant Violet 711™ anti-human CD15 (SSEA-1)	W6D3	FC
APC/Cyanine7 anti-human CD15 (SSEA-1)	W6D3	FC
TotalSeq™-B0392 anti-human CD15 (SSEA-1)	W6D3	PG
TotalSeq™-C0392 anti-human CD15 (SSEA-1)	W6D3	PG
PE anti-human CD15 (SSEA-1)	W6D3	FC
APC/Fire™ 810 anti-human CD15 (SSEA-1) Antibody	W6D3	FC
TotalSeq™-D0392 anti-human CD15 (SSEA-1)	W6D3	PG
GMP FITC anti-human CD15 (SSEA-1)	W6D3	FC
Spark YG™ 593 anti-human CD15 (SSEA-1)	W6D3	FC
GMP PE anti-human CD15 (SSEA-1)	W6D3	FC
GMP Pacific Blue™ anti-human CD15 (SSEA-1)	W6D3	FC
Spark Violet™ 500 anti-human CD15 (SSEA-1)	W6D3	FC
Spark UV™ 387 anti-human CD15 (SSEA-1)	W6D3	FC
PE/Cyanine7 anti-human CD15 (SSEA-1)	W6D3	FC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 07/22/2022

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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