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Purified anti-human CD150 (SLAM) Antibody

Pricing & Availability
Clone
A12 (7D4) (See other available formats)
Regulatory Status
RUO
Other Names
SLAM, Signaling Lymphocyte Activation Molecule, IPO-3
Isotype
Mouse IgG1, κ
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Product Citations
publications
A12_Pure_021009.jpg
Human peripheral blood lymphocytes stained with purified A12, followed by biotinylated anti-mouse IgG and Sav-PE
  • A12_Pure_021009.jpg
    Human peripheral blood lymphocytes stained with purified A12, followed by biotinylated anti-mouse IgG and Sav-PE
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306302 100 µg $101
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Description

CD150 is a 70-95 kD type I transmembrane glycoprotein also known as SLAM or IPO-3. It is a member of the Ig superfamily. It is expressed on a subset of T cells, B cells, dendritic cells, and endothelial cells. The expression of CD150 is upregulated upon activation. CD150 binds to itself as the ligand to be involved in B cell costimulation, proliferation, immunoglobulin production, and signal transduction.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Activated human PBMC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, Costim, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation4, and costimulation1,5 of IFN-gamma production and T cell proliferation. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306317 and 306318).

Application References

(PubMed link indicates BioLegend citation)
  1. Garcia V, et al. 2001. J. Immunol. 167:5719. (Costim)
  2. Vincent S, et al. 2002. J. Virol. 76:6121.
  3. Cocks B, et al. 1995. Nature 376:260.
  4. Sayos J, et al. 2001. Blood 97:3867. (IP)
  5. Aversa G, et al. 1997. J. Immunol. 158:4036. (Costim)
  6. Spencer M, et al. 2010. Am. J. Physiol Endocrinol Metab. 299:1016. PubMed
Product Citations
  1. Baldanzi G, et al. 2011. J Immunol. 187:5941. PubMed
  2. Spencer M, et al. 2010. Am J Physiol Endocrinol Metab. 299:E1016. PubMed
RRID
AB_314590 (BioLegend Cat. No. 306302)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 70-95 kD
Distribution

T subset (upregulated after activation), B cells, dendritic cells, endothelial cells

Function
B cell costimulation, proliferation and Ig production
Ligand/Receptor
CD150 (self ligand)
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks B, et al. 1995. Nature 376:260.
2. Pinchouk V, et al. 1988. AntiCancer Res. 8:1377.
3. Polacino P, et al. 1996. J. Med. Primatol. 25:201.
4. Punnonen J, et al. 1997. J. Exp. Med. 185:993.

Gene ID
6504 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org
Go To Top Version: 2    Revision Date: 08/15/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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